Detailed information of TA system TA143796

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 301743..302339
• Replicon: chromosome
• Accession: NZ_CP046271
• Organism: Enterobacter hormaechei strain E70

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A837F9W8
• Locus tag: GMA21_RS01440
• Protein ID: WP_023315213.1
• Coordinates: 302037..302339 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: GMA21_RS01435
• Protein ID: WP_023315212.1
• Coordinates: 301743..302030 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GMA21_RS01430	300115..301746	+	1632	WP_058652456.1	Na/Pi cotransporter family protein	-
GMA21_RS01435	301743..302030	-	288	WP_023315212.1	putative addiction module antidote protein	Antitoxin
GMA21_RS01440	302037..302339	-	303	WP_023315213.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GMA21_RS01445	302537..303409	+	873	WP_045346556.1	23S rRNA pseudouridine(2604) synthase RluF	-
GMA21_RS01450	303410..303682	-	273	WP_003860346.1	DUF3811 domain-containing protein	-
GMA21_RS01455	303733..304677	-	945	WP_058652454.1	ketopantoate/pantoate/pantothenate transporter PanS	-
GMA21_RS01460	304771..306120	-	1350	WP_003860344.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11427.21 Da
• Isoelectric Point: 10.1042

>T143796 WP_023315213.1 NZ_CP046271:c302339-302037 [Enterobacter hormaechei]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRVYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T143796 NZ_CP061368:c1689551-1689297 [Wenzhouxiangella sp. AB-CW3]
GTGCTGGCCAAGTTCTACGAAACCGGTTCAGTAGCAGGAGTTCAACCAAGCCATGCCAAACGTCTGCGAATGTTACTGGC
AGCCTTGGATACCGCCGCGTCTATCCAAGATATGGATGTGCCAGGGTTCCGGTTGCACCGTCTCAAGGGAATGGACGCTG
ATCGGTGGTCAATCCGTGTGAGTGAAAATTGGAGGCTGACCTATGAGTTTAGAGATGGACAGGCCCACGTTGTTGACTAC
GAGGACTATCACTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10171.73 Da
• Isoelectric Point: 7.0086

>AT143796 WP_023315212.1 NZ_CP046271:c302030-301743 [Enterobacter hormaechei]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISLQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLRLTIKPSGD

Nucleotide

• Length: 288 bp

>AT143796 NZ_CP061368:c1689297-1689004 [Wenzhouxiangella sp. AB-CW3]
ATGTCTATGCATAATCCCCCTCATCCCGGAGAATTCATCCGTGAGGTTTACCTAGAGCCTCATTCAATGACCGGACGTCA
GCTGGCTTCGAAGCTGTGTGTTTCGCCGTCCACTTTGAATCGTATCTTGCAGGAACGAAGTGGAATTAGTCCGGAAATGG
CGCTTCGCCTATCCAAAGCTCTTGGTCGTTCGCCGGAAAGCTGGTTGCTGATGCAAGACAACTACGAGCTCTGGCATGCT
CGACAAACTGTAGATCTAGAATCTGTCGAAAAAGTTGAGTTCCGTGCAGCCTAA

Structures

Toxin

• AlphaFold DB: A0A837F9W8