Detailed information of TA system TA143790

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 2598148..2598799
• Replicon: chromosome
• Accession: NZ_CP046267
• Organism: Lacticaseibacillus rhamnosus strain BIO5326

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: K8Q4Y0
• Locus tag: GMB04_RS12555
• Protein ID: WP_005686631.1
• Coordinates: 2598148..2598531 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: K8QH19
• Locus tag: GMB04_RS12560
• Protein ID: WP_005686632.1
• Coordinates: 2598551..2598799 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GMB04_RS12540	2597107..2597634	-	528	WP_005691221.1	QueT transporter family protein	-
GMB04_RS12555	2598148..2598531	-	384	WP_005686631.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GMB04_RS12560	2598551..2598799	-	249	WP_005686632.1	antitoxin	Antitoxin
GMB04_RS12565	2598911..2600050	-	1140	WP_005691215.1	alanine racemase	-
GMB04_RS12570	2600037..2600411	-	375	WP_005686634.1	holo-ACP synthase	-
GMB04_RS12575	2600580..2602088	-	1509	WP_005686635.1	DEAD/DEAH box helicase	-
GMB04_RS12580	2602362..2603750	-	1389	WP_014571607.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13757.03 Da
• Isoelectric Point: 10.9764

>T143790 WP_005686631.1 NZ_CP046267:c2598531-2598148 [Lacticaseibacillus rhamnosus]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Nucleotide

• Length: 384 bp

>T143790 NZ_CP061367:4632217-4632495 [Shigella sonnei]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8936.13 Da
• Isoelectric Point: 4.4778

>AT143790 WP_005686632.1 NZ_CP046267:c2598799-2598551 [Lacticaseibacillus rhamnosus]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Nucleotide

• Length: 249 bp

>AT143790 NZ_CP061367:4631954-4632214 [Shigella sonnei]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCTGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: K8Q4Y0

Antitoxin

• AlphaFold DB: K8QH19