Detailed information of TA system TA143787

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 222964..223600
• Replicon: chromosome
• Accession: NZ_CP046266
• Organism: Bacillus sp. DSL-17

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0M2PN83
• Locus tag: GMB29_RS01220
• Protein ID: WP_026562078.1
• Coordinates: 223250..223600 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: GMB29_RS01215
• Protein ID: WP_136358446.1
• Coordinates: 222964..223245 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GMB29_RS01195	218475..219197	-	723	WP_136358454.1	rhomboid family intramembrane serine protease	-
GMB29_RS01200	219358..219720	+	363	WP_136358452.1	holo-ACP synthase	-
GMB29_RS01205	220064..221086	+	1023	WP_136358450.1	outer membrane lipoprotein carrier protein LolA	-
GMB29_RS01210	221430..222599	+	1170	WP_136358448.1	alanine racemase	-
GMB29_RS01215	222964..223245	+	282	WP_136358446.1	antitoxin endoai	Antitoxin
GMB29_RS01220	223250..223600	+	351	WP_026562078.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
GMB29_RS01225	223737..224567	+	831	WP_136358444.1	STAS domain-containing protein	-
GMB29_RS01230	224571..224933	+	363	WP_136358442.1	STAS domain-containing protein	-
GMB29_RS01235	224937..225338	+	402	WP_136358440.1	anti-sigma regulatory factor	-
GMB29_RS01240	225348..226355	+	1008	WP_136358438.1	PP2C family protein-serine/threonine phosphatase	-
GMB29_RS01245	226412..226744	+	333	WP_136358436.1	anti-sigma factor antagonist	-
GMB29_RS01250	226741..227223	+	483	WP_136358434.1	anti-sigma B factor RsbW	-
GMB29_RS01255	227189..227980	+	792	WP_136358432.1	RNA polymerase sigma factor SigB	-
GMB29_RS01260	227977..228576	+	600	WP_136358430.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13020.06 Da
• Isoelectric Point: 4.8781

>T143787 WP_026562078.1 NZ_CP046266:223250-223600 [Bacillus sp. DSL-17]
MIVKRGDVYFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLGLIDF

Nucleotide

• Length: 351 bp

>T143787 NZ_CP061367:c3559985-3559882 [Shigella sonnei]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTTTTTTT

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10559.02 Da
• Isoelectric Point: 5.1783

>AT143787 WP_136358446.1 NZ_CP046266:222964-223245 [Bacillus sp. DSL-17]
VSESSATTEILIQLPQALVSELDGLVKQENGNRNELIYQATKMYIRERKMRQIRESMRRGYMEMAKINLNIASEAFLAES
EADHTVERLVSGG

Nucleotide

• Length: 282 bp

>AT143787 NZ_CP061367:3559881-3560115 [Shigella sonnei]
TAAAAAAAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCA
TTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAAT
AAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCGTTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A0M2PN83