Detailed information of TA system TA143775

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3924290..3925127
• Replicon: chromosome
• Accession: NZ_CP046259
• Organism: Escherichia coli strain ECO2947

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: GK046_RS18810
• Protein ID: WP_000227784.1
• Coordinates: 3924290..3924832 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: GK046_RS18815
• Protein ID: WP_001297137.1
• Coordinates: 3924816..3925127 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GK046_RS18790	3919829..3920740	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
GK046_RS18795	3920908..3921399	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
GK046_RS18800	3921527..3922891	-	1365	WP_001000974.1	MFS transporter	-
GK046_RS18805	3923299..3924234	+	936	WP_001297127.1	sel1 repeat family protein	-
GK046_RS18810	3924290..3924832	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
GK046_RS18815	3924816..3925127	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
GK046_RS18820	3925312..3926202	-	891	WP_000971336.1	heme o synthase	-
GK046_RS18825	3926214..3926543	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
GK046_RS18830	3926543..3927157	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
GK046_RS18835	3927147..3929138	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
GK046_RS18840	3929160..3930107	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T143775 WP_000227784.1 NZ_CP046259:c3924832-3924290 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T143775 NZ_CP061352:36974-37279 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT143775 WP_001297137.1 NZ_CP046259:c3925127-3924816 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT143775 NZ_CP061352:36754-36972 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3