Detailed information of TA system TA143773

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3346786..3348469
• Replicon: chromosome
• Accession: NZ_CP046259
• Organism: Escherichia coli strain ECO2947

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: GK046_RS16170
• Protein ID: WP_125090762.1
• Coordinates: 3346786..3347703 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: I2UPX5
• Locus tag: GK046_RS16175
• Protein ID: WP_000157137.1
• Coordinates: 3347696..3348469 (-)
• Length: 258 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GK046_RS16130	3342319..3342540	+	222	WP_000692311.1	DUF987 domain-containing protein	-
GK046_RS16135	3342703..3343077	+	375	WP_001280952.1	type IV toxin-antitoxin system YeeU family antitoxin	-
GK046_RS16140	3343124..3343501	+	378	WP_001506713.1	TA system toxin CbtA family protein	-
GK046_RS16145	3343498..3343986	+	489	WP_001470575.1	hypothetical protein	-
GK046_RS16150	3343998..3344195	+	198	WP_000839249.1	DUF957 domain-containing protein	-
GK046_RS24780	3344274..3344432	+	159	WP_001467148.1	hypothetical protein	-
GK046_RS16160	3344896..3345606	+	711	WP_001219054.1	integrase arm-type DNA-binding domain-containing protein	-
GK046_RS16165	3345610..3346272	+	663	WP_000357928.1	hypothetical protein	-
GK046_RS24785	3346325..3346492	-	168	WP_001356018.1	hypothetical protein	-
GK046_RS16170	3346786..3347703	-	918	WP_125090762.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
GK046_RS16175	3347696..3348469	-	774	WP_000157137.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
GK046_RS16180	3348553..3348873	+	321	Protein_3147	IS3 family transposase	-
GK046_RS16185	3349049..3350398	-	1350	WP_125090760.1	phosphatidylserine/phosphatidylglycerophosphate/ cardiolipin synthase family protein	-
GK046_RS16190	3350517..3352601	-	2085	WP_001193075.1	protease Lon-related BREX system protein BrxL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (13-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34963.09 Da
• Isoelectric Point: 4.8470

>T143773 WP_125090762.1 NZ_CP046259:c3347703-3346786 [Escherichia coli]
MDKTSPYYRQVALLVRALPWVANESCFALKGGTAINLFIHDFPRLSVDIDLAWIPLESREVALPNVREALTRIAAELEAQ
TGIRAVLKTHNPDEMRIIVTTDDAQIKIEVSPVARGTLYPPQERDIVGAVEEEFGFAALPVVSLPDLYGGKLCAALDRQH
PRDFYDVKMLLDTQGIDRSIFNGFIVYLLSHNRPLSEVLNPRWKDITEPFQNEFSGMTFEEVSLEELKEVPERMLSTLKT
HFTQQDFDFLMSFKRGEPDWNLAPEPHIQNLPAVQWKLRNIGQMSEEKRAQSLEKLEKVMTEWLR

Nucleotide

• Length: 918 bp

>T143773 NZ_CP061351:53390-53872 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCACTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 258 a.a.
• Molecular weight: 29333.76 Da
• Isoelectric Point: 9.9889

>AT143773 WP_000157137.1 NZ_CP046259:c3348469-3347696 [Escherichia coli]
MASKLNWLLQNTSPGSLVLQSWLTRNNISPSLAFKYAQNGWLIKLRAGVYARAGREPDWSDALWCLQNQLEAPVHLAGLS
SLSWQGRSHYLQLKQNQCWLSVENKAILPKWFKEFPAVEWLMVSALKLPDLDEKYRTELEVKGKRITASTPELAAYELLS
AVPQEISFEHAAELFQGLVNLNPRKVEKLLTLSQSVQTKRLYLFFASFYEHAWFKRIDTSKINLGSGNRQIVVNGKLNTQ
YQITVPENFTKKGISHG

Nucleotide

• Length: 774 bp

>AT143773 NZ_CP061351:53136-53402 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGTACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0H0N3W2