Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3148204..3148701 Replicon chromosome
Accession NZ_CP046259
Organism Escherichia coli strain ECO2947

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag GK046_RS15095 Protein ID WP_001173343.1
Coordinates 3148528..3148701 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag GK046_RS15090 Protein ID WP_000398619.1
Coordinates 3148204..3148500 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GK046_RS15070 3144935..3145654 - 720 WP_125090678.1 two-component system response regulator DcuR -
GK046_RS15075 3145651..3147282 - 1632 WP_125090680.1 sensor histidine kinase -
GK046_RS15080 3147463..3147693 + 231 WP_000371700.1 (4Fe-4S)-binding protein -
GK046_RS15085 3147705..3147977 + 273 WP_000405647.1 N-acetyltransferase -
GK046_RS15090 3148204..3148500 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
GK046_RS15095 3148528..3148701 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
GK046_RS15100 3148820..3150337 - 1518 WP_001295074.1 lysine--tRNA ligase -
GK046_RS15105 3150574..3152031 - 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T143769 WP_001173343.1 NZ_CP046259:3148528-3148701 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T143769 NZ_CP061350:c3602973-3602871 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT143769 WP_000398619.1 NZ_CP046259:3148204-3148500 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT143769 NZ_CP061350:3602864-3603009 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References