Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1793183..1793837 Replicon chromosome
Accession NZ_CP046259
Organism Escherichia coli strain ECO2947

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag GK046_RS08675 Protein ID WP_000244772.1
Coordinates 1793183..1793590 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GK046_RS08680 Protein ID WP_000354046.1
Coordinates 1793571..1793837 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GK046_RS08655 1789140..1790873 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
GK046_RS08660 1790879..1791589 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GK046_RS08665 1791614..1792510 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
GK046_RS08670 1792622..1793143 + 522 WP_001055874.1 flavodoxin FldB -
GK046_RS08675 1793183..1793590 - 408 WP_000244772.1 protein YgfX Toxin
GK046_RS08680 1793571..1793837 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GK046_RS08685 1794080..1795060 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
GK046_RS08690 1795256..1795915 - 660 WP_000250274.1 hemolysin III family protein -
GK046_RS08695 1796079..1796390 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
GK046_RS08700 1796435..1797868 + 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
GK046_RS08705 1797925..1798668 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T143765 WP_000244772.1 NZ_CP046259:c1793590-1793183 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T143765 NZ_CP061350:c917843-916908 [Klebsiella pneumoniae]
CTGCTTTTTTCTGATGTAACAGATGCTCTTGGTATTGAGTCCGGCGCTATTGTAGAAAAAGATCACTATATCGTTGAGCT
TTTACATTTACTGAAACCGCTAACATTTGACACTCATCAATTGCTGTTTGCCGGAGGAACTGCACTGGCAAAATCGGGCA
TTGTGCTTAACCGAATGTCGGAAGATGTAGATATAAAACTGGTACCCACAGATACTTTCTTGTCTCTTTCGCGCACCCAG
CGCAAAGGTATTCGTAAAGAGATCGTCCAGACAATTTCTCATGCAATAACTGCAACAGACATGTTCAGCTTTGACGATGC
CCATCCAAAAGTGACGCGGGATGAATATCGTTACAACGACATACCCGTTCGCTATCCGCAGCGGGTAGCGCAAGCACCTT
GCCTGCGTCCTTTTATCCAGTTGGAATTGATGGAAGCAAATTTGCTTGAGGCACCAGAGTTCAGGGATATTCATACACTG
GTGACGACACTTACTGGAAAAGCACCTGTCATTTCAGCCTTCCCCTGCGCCACAATTATCAGTACTCAGGCAGAAAAACT
GGTGGCTATGCTGCGCCGTACTGCAGCAGTTGCGCGTAATATTGAACGCCAGGATGACGCTTCGCTGGTACGTCATATCT
ACGACACATATTGTATCGTGCAGGCAGGCAATGTTGATATGTGTTTGCTTACTGATTTTTTTAAGCAAACCATTGAGCAA
GATATCCAGCGTTATGGCAATCAGTATCCGCAGTTTTGTGTTTCACCAAATGATGAGCTAAAAATGGGGCTGGACGAACT
TGAGCATAACCCGCTTTATAAGACACGCTACCAGCAATTTGTTGCCCCAATGGTATATGGTGCTCGCAAGGTATCGTGGG
ATGAAGCGTATGCTTCCTTTCGTCAAATGGCGTTAATAATTCTAGATACTTACTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT143765 WP_000354046.1 NZ_CP046259:c1793837-1793571 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT143765 NZ_CP061350:c918245-917850 [Klebsiella pneumoniae]
ATGACGATAAAAACACGCATTCAGTCACGACTGAAACGCTCAAAACGCTATGTATTCACTCGTGACGATTTCAAGGATAT
TGCAGGCTATGATCAGATTGGCAGGGTATTGCGCGACCTTCTCAAAGAGGGGCAACTACTGAAGGTGGGCTACGGTGTGT
ACACCAAAGCGCGTCGGAACAGCATTACTGGAAAAGTCATGCCCGCAGCACCAAGGGGATCTTCGGCAGTGATCGTTGAA
ACGCTGGAGCGCCTGAAAGTACGTTACCGCTATGAAGGAGCTACACAGACATATAATAGTGGCGAATCCACGCAAATCCC
CGTGTCACCGGAGATCAAAACATCTCCGCGCTTTAAACGCGCGTTGTCTGTGGGGAACAGCAAACTAAATGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References