Detailed information of TA system TA143754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 273289..274877
• Replicon: chromosome
• Accession: NZ_CP046259
• Organism: Escherichia coli strain ECO2947

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A5F1UYE1
• Locus tag: GK046_RS01250
• Protein ID: WP_001125468.1
• Coordinates: 273289..274611 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GK046_RS01255
• Protein ID: WP_001296726.1
• Coordinates: 274611..274877 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GK046_RS01235	270703..271413	-	711	WP_001195165.1	fimbria/pilus periplasmic chaperone	-
GK046_RS01240	271774..272337	-	564	WP_001296758.1	type 1 fimbrial major subunit FimA	-
GK046_RS01245	272769..272977	-	209	Protein_248	helix-turn-helix transcriptional regulator	-
GK046_RS01250	273289..274611	-	1323	WP_001125468.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
GK046_RS01255	274611..274877	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49408.03 Da
• Isoelectric Point: 9.1203

>T143754 WP_001125468.1 NZ_CP046259:c274611-273289 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKEVGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T143754 NZ_CP061349:c1582488-1582243 [Staphylococcus aureus]
ATGAACATTCAAGAAGCAACTAAGATAGCTACAAAAAATCTTGTCTCTATGACACGGAAAGATTGGAAAGAAAGTCATCG
AACTAAGATATTACCAACAAATGATAGTTTTTTACAATGCATCATTTCAAATAGCGATGGGACAAACCTTATCAGATATT
GGCAACCTTCAGCCGATGACCTCATGGCAAATGATTGGGAAGTTATAAACCCAACTAGAGACCAGGAATTATTGAAGCAA
TTTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT143754 WP_001296726.1 NZ_CP046259:c274877-274611 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT143754 NZ_CP061349:1581909-1582274 [Staphylococcus aureus]
ATGCCCTTGATATCTGATGAATTTGATACACTTACTAAAGACCAACAATATATCTTGTCCGTACTCTACAAAGATTATTT
AGAATGTGTAAAGTTAGGTTCGGTTAAATTAACCTGCAATAATTTTGGAAGTGCTAAAGATATACATACAAAGTATTTTC
AAAAACTACATTTCGAAGATGTAAAATACGATTTAAATAAACTTAAAAACTCTGGGTTCCTAAACGGCGTGTATGCTAGT
AACACTATTTATCATGTAACAATTTCAGACAAGACTGTTGTTTACTTTGAAAATGAGTTTAAAAACAATTTAAAAAGTAT
CATTGATAGCATTTCTAAAATTGCTTCAATAATTCCTGGTCTCTAG

Structures

Toxin

• AlphaFold DB: A0A5F1UYE1