Detailed information of TA system TA143752

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 516214..516872
• Replicon: chromosome
• Accession: NZ_CP046253
• Organism: Sphingobium sp. CAP-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A084EIW7
• Locus tag: GL174_RS16760
• Protein ID: WP_037520637.1
• Coordinates: 516435..516872 (+)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GL174_RS16755
• Protein ID: WP_196221871.1
• Coordinates: 516214..516438 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GL174_RS16725	512080..512346	+	267	WP_037520626.1	hypothetical protein	-
GL174_RS16730	512423..512698	+	276	WP_037520628.1	HU family DNA-binding protein	-
GL174_RS16735	513295..514053	+	759	WP_037520629.1	LuxR family transcriptional regulator	-
GL174_RS16740	514192..514806	+	615	WP_037520631.1	autoinducer synthase	-
GL174_RS16745	514818..515717	+	900	WP_037520632.1	phytanoyl-CoA dioxygenase family protein	-
GL174_RS16750	515760..516182	+	423	WP_037520635.1	MarR family winged helix-turn-helix transcriptional regulator	-
GL174_RS16755	516214..516438	+	225	WP_196221871.1	CopG family transcriptional regulator	Antitoxin
GL174_RS16760	516435..516872	+	438	WP_037520637.1	PIN domain-containing protein	Toxin
GL174_RS16765	516872..517792	+	921	WP_037520638.1	HEPN domain-containing protein	-
GL174_RS16770	518089..518385	-	297	WP_037520732.1	hypothetical protein	-
GL174_RS16775	518511..518858	-	348	WP_051886785.1	hypothetical protein	-
GL174_RS16780	518907..520160	-	1254	WP_037520733.1	hypothetical protein	-
GL174_RS16785	520286..520864	-	579	WP_037520641.1	PIN domain-containing protein	-
GL174_RS16790	520861..521310	-	450	WP_037520643.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	500852..534926	34074

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-127)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15628.87 Da
• Isoelectric Point: 6.7650

>T143752 WP_037520637.1 NZ_CP046253:516435-516872 [Sphingobium sp. CAP-1]
MTYLLDVNVLVALIDPNHVGHEAAHHWFETEGGKAWATCPITENGVIRIVGNVRYPSTPGSPAEVASIMAQMCKLPGHRF
WAEDISLLSAEHVDASHILTSAQVTDTYLLALAVANKGKLATFDRRLTPKGVTGGKDAIHLIGNH

Nucleotide

• Length: 438 bp

>T143752 NZ_CP061349:c810439-810251 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7908.05 Da
• Isoelectric Point: 7.3199

>AT143752 WP_196221871.1 NZ_CP046253:516214-516438 [Sphingobium sp. CAP-1]
MRTTLVIDDDVMAAARAIADHQHSSIGRVLSDLARKALHAPEAARTRNGISLLPTKPGVVVTQDIVNALRDEAP

Nucleotide

• Length: 225 bp

>AT143752 NZ_CP061349:c810728-810468 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: A0A084EIW7