Detailed information of TA system TA143750

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4183562..4184241
• Replicon: chromosome
• Accession: NZ_CP046251
• Organism: Leclercia adecarboxylata strain P12375

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: GLX29_RS19740
• Protein ID: WP_155167339.1
• Coordinates: 4183562..4183903 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: GLX29_RS19745
• Protein ID: WP_155167341.1
• Coordinates: 4183924..4184241 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GLX29_RS19720	4179601..4181448	+	1848	WP_032614248.1	RNA polymerase sigma factor RpoD	-
GLX29_RS19725	4181803..4182309	-	507	WP_032614250.1	G/U mismatch-specific DNA glycosylase	-
GLX29_RS19735	4182614..4183447	-	834	WP_155167337.1	DUF4942 domain-containing protein	-
GLX29_RS19740	4183562..4183903	-	342	WP_155167339.1	TA system toxin CbtA family protein	Toxin
GLX29_RS19745	4183924..4184241	-	318	WP_155167341.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GLX29_RS19750	4184260..4184481	-	222	WP_155167343.1	DUF987 domain-containing protein	-
GLX29_RS19755	4184490..4184966	-	477	WP_155167345.1	RadC family protein	-
GLX29_RS19760	4184982..4185440	-	459	WP_155167347.1	antirestriction protein	-
GLX29_RS19765	4185537..4185776	-	240	WP_155167349.1	DUF905 domain-containing protein	-
GLX29_RS19770	4185855..4186328	-	474	WP_155167351.1	hypothetical protein	-
GLX29_RS19775	4186350..4187189	-	840	WP_155167353.1	hypothetical protein	-
GLX29_RS19780	4187228..4187929	-	702	WP_155167355.1	WYL domain-containing protein	-
GLX29_RS19785	4188146..4188967	-	822	WP_155167357.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4183562..4208451	24889

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12779.74 Da
• Isoelectric Point: 8.5073

>T143750 WP_155167339.1 NZ_CP046251:c4183903-4183562 [Leclercia adecarboxylata]
MKPQPAATSRAVKPCLSPVVVWQMLLTRLLEKHYGLTLNDTPFSDDTVIQEHINAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRAQQATGLLRQSCNNAVR

Nucleotide

• Length: 342 bp

>T143750 NZ_CP061340:136698-136985 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGGCTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCTTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGCGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12001.66 Da
• Isoelectric Point: 6.8412

>AT143750 WP_155167341.1 NZ_CP046251:c4184241-4183924 [Leclercia adecarboxylata]
MGNITWGLQRDITPRLGARLVQEGNRLHYLADRASITGKFSDPECRKLDETFPHFISQMELMLTTGELNPRYTHCITLYH
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT143750 NZ_CP061340:136450-136701 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAACCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TTGATGCCCTAGACGATCAGGAGTTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGATCTGGAT
AAATACCTATGA