Detailed information of TA system TA143747

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 3627481..3628214
• Replicon: chromosome
• Accession: NZ_CP046251
• Organism: Leclercia adecarboxylata strain P12375

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: GLX29_RS17165
• Protein ID: WP_155167160.1
• Coordinates: 3627885..3628214 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: GLX29_RS17160
• Protein ID: WP_060617827.1
• Coordinates: 3627481..3627846 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GLX29_RS17130	3623478..3623783	+	306	WP_155167154.1	hypothetical protein	-
GLX29_RS17135	3623827..3624708	+	882	WP_155167155.1	hypothetical protein	-
GLX29_RS17140	3625383..3626207	+	825	WP_155167156.1	DUF945 domain-containing protein	-
GLX29_RS17145	3626239..3626682	+	444	WP_155167157.1	antirestriction protein	-
GLX29_RS17150	3626695..3627237	+	543	WP_155167158.1	DNA repair protein RadC	-
GLX29_RS17155	3627234..3627455	+	222	WP_155167159.1	DUF987 domain-containing protein	-
GLX29_RS17160	3627481..3627846	+	366	WP_060617827.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GLX29_RS17165	3627885..3628214	+	330	WP_155167160.1	TA system toxin CbtA family protein	Toxin
GLX29_RS17170	3628531..3628821	-	291	WP_167540286.1	antibiotic biosynthesis monooxygenase	-
GLX29_RS17175	3628838..3629839	-	1002	WP_032613327.1	zinc-binding alcohol dehydrogenase family protein	-
GLX29_RS17180	3629948..3630850	+	903	WP_059307360.1	LysR family transcriptional regulator	-
GLX29_RS17185	3630921..3631775	+	855	WP_059309077.1	kinase	-
GLX29_RS17190	3631819..3632283	-	465	WP_155167161.1	YhcH/YjgK/YiaL family protein	-
GLX29_RS17195	3632280..3633155	-	876	WP_155167162.1	N-acetylmannosamine kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3612995..3628836	15841

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12378.18 Da
• Isoelectric Point: 7.2414

>T143747 WP_155167160.1 NZ_CP046251:3627885-3628214 [Leclercia adecarboxylata]
MQTLSSYPTRAAQPCLSPIEIWQCLLPHLLSQHYGLTLNDTPFSNETTIQEHIDAGISLSDAVNFLVEKYGLVRIDRKGF
SWQEQTPYLSVVDILRARRSTGLLKTNMK

Nucleotide

• Length: 330 bp

>T143747 NZ_CP061340:26131-26436 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCTTGCTGTCAGATAAAGTCTCCCGTGAACTTTATCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCTGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGGGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13498.22 Da
• Isoelectric Point: 6.4731

>AT143747 WP_060617827.1 NZ_CP046251:3627481-3627846 [Leclercia adecarboxylata]
MNNHSESGTKPDNPACQQWGLKRTITPCFGARLVQEGNRVHFLADRAGFNGAFSDGDVLHLDQAFPLILKQLELMLTSGK
LNPRYQHCVTFYHNGLTCEADTLGSCGYIYIAIYPDQTEPH

Nucleotide

• Length: 366 bp

>AT143747 NZ_CP061340:25911-26129 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGCGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA