Detailed information of TA system TA143738

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2334559..2335133
• Replicon: chromosome
• Accession: NZ_CP046251
• Organism: Leclercia adecarboxylata strain P12375

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GLX29_RS10970
• Protein ID: WP_103792339.1
• Coordinates: 2334807..2335133 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A6G7TED5
• Locus tag: GLX29_RS10965
• Protein ID: WP_059307895.1
• Coordinates: 2334559..2334807 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GLX29_RS10950	2331462..2333006	+	1545	WP_059307898.1	nitrate reductase subunit beta	-
GLX29_RS10955	2333006..2333701	+	696	WP_155166791.1	nitrate reductase molybdenum cofactor assembly chaperone	-
GLX29_RS10960	2333698..2334378	+	681	WP_032618185.1	respiratory nitrate reductase subunit gamma	-
GLX29_RS10965	2334559..2334807	+	249	WP_059307895.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GLX29_RS10970	2334807..2335133	+	327	WP_103792339.1	endoribonuclease MazF	Toxin
GLX29_RS10975	2335222..2335671	-	450	WP_144837973.1	hypothetical protein	-
GLX29_RS10980	2335905..2336750	-	846	WP_059307893.1	N-hydroxyarylamine O-acetyltransferase	-
GLX29_RS10985	2336947..2338602	-	1656	WP_103792337.1	iron ABC transporter permease	-
GLX29_RS10990	2338641..2339609	-	969	WP_117386488.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-107)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11861.62 Da
• Isoelectric Point: 7.8831

>T143738 WP_103792339.1 NZ_CP046251:2334807-2335133 [Leclercia adecarboxylata]
MDYIPEAGDIVWLDFDPQSGHEQAGHRPALVVSPATYNGRTGLMLCCPVTSQIKNYPFEVVISGARRNVALADQIKSLDW
RARHAVKKGTATAEELDAVRMRAKVLIG

Nucleotide

• Length: 327 bp

>T143738 NZ_CP061339:2135461-2135637 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9137.39 Da
• Isoelectric Point: 4.4777

>AT143738 WP_059307895.1 NZ_CP046251:2334559-2334807 [Leclercia adecarboxylata]
MPQVTVKKWGNSPSVRLPVAVMREAELHVDDVVNVNVDEEGRIILTPVRKDEPTLESLLAAITDDNLHHEVTFGEPQGKE
LL

Nucleotide

• Length: 249 bp

>AT143738 NZ_CP061339:2135683-2136099 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A6G7TED5