Detailed information of TA system TA143730

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2472293..2472864
• Replicon: chromosome
• Accession: NZ_CP046247
• Organism: Enterococcus faecalis strain RF_1_1_25_1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GLO19_RS11810
• Protein ID: WP_243513340.1
• Coordinates: 2472293..2472634 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: GLO19_RS11815
• Protein ID: WP_002354773.1
• Coordinates: 2472634..2472864 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GLO19_RS11805 (2468308)	2468308..2471922	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
GLO19_RS11810 (2472293)	2472293..2472634	-	342	WP_243513340.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GLO19_RS11815 (2472634)	2472634..2472864	-	231	WP_002354773.1	hypothetical protein	Antitoxin
GLO19_RS11820 (2473038)	2473038..2473253	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
GLO19_RS11825 (2473392)	2473392..2474384	+	993	WP_002378862.1	biotin--[acetyl-CoA-carboxylase] ligase	-
GLO19_RS11830 (2474551)	2474551..2475189	-	639	WP_002378863.1	lytic polysaccharide monooxygenase	-
GLO19_RS11835 (2475874)	2475874..2477490	+	1617	WP_002372620.1	phosphatase PAP2/LCP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13142.33 Da
• Isoelectric Point: 8.8646

>T143730 WP_243513340.1 NZ_CP046247:c2472634-2472293 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQNRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T143730 NZ_CP061339:374664-375014 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT143730 WP_002354773.1 NZ_CP046247:c2472864-2472634 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT143730 NZ_CP061339:374419-374670 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATTTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812