Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ImmA-IrrE/HTH_19(antitoxin) |
| Location | 2170027..2171060 | Replicon | chromosome |
| Accession | NZ_CP046247 | ||
| Organism | Enterococcus faecalis strain RF_1_1_25_1 | ||
Toxin (Protein)
| Gene name | IrrE | Uniprot ID | E2YSW3 |
| Locus tag | GLO19_RS10450 | Protein ID | WP_002380879.1 |
| Coordinates | 2170407..2171060 (+) | Length | 218 a.a. |
Antitoxin (Protein)
| Gene name | ImmA | Uniprot ID | - |
| Locus tag | GLO19_RS10445 | Protein ID | WP_002380877.1 |
| Coordinates | 2170027..2170410 (+) | Length | 128 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| GLO19_RS10400 (2166363) | 2166363..2166584 | - | 222 | WP_225589883.1 | DUF86 domain-containing protein | - |
| GLO19_RS10405 (2166744) | 2166744..2167070 | - | 327 | WP_002394268.1 | nucleotidyltransferase domain-containing protein | - |
| GLO19_RS10410 (2167130) | 2167130..2167423 | - | 294 | WP_029655860.1 | hypothetical protein | - |
| GLO19_RS10415 (2167465) | 2167465..2167617 | - | 153 | WP_002371433.1 | hypothetical protein | - |
| GLO19_RS10420 (2167844) | 2167844..2168032 | + | 189 | WP_002371434.1 | hypothetical protein | - |
| GLO19_RS10425 (2168019) | 2168019..2168180 | - | 162 | WP_002380875.1 | hypothetical protein | - |
| GLO19_RS10430 (2168203) | 2168203..2168619 | - | 417 | WP_002380876.1 | DUF961 family protein | - |
| GLO19_RS10435 (2168619) | 2168619..2168945 | - | 327 | WP_002365226.1 | hypothetical protein | - |
| GLO19_RS10440 (2169031) | 2169031..2169282 | - | 252 | WP_002365227.1 | hypothetical protein | - |
| GLO19_RS10445 (2170027) | 2170027..2170410 | + | 384 | WP_002380877.1 | helix-turn-helix transcriptional regulator | Antitoxin |
| GLO19_RS10450 (2170407) | 2170407..2171060 | + | 654 | WP_002380879.1 | ImmA/IrrE family metallo-endopeptidase | Toxin |
| GLO19_RS10455 (2171099) | 2171099..2171434 | + | 336 | WP_002399532.1 | helix-turn-helix domain-containing protein | - |
| GLO19_RS10460 (2171472) | 2171472..2172803 | - | 1332 | WP_010712813.1 | FAD-dependent oxidoreductase | - |
| GLO19_RS10465 (2172902) | 2172902..2173777 | - | 876 | WP_225589882.1 | aldo/keto reductase | - |
| GLO19_RS10470 (2173853) | 2173853..2174272 | - | 420 | WP_010712815.1 | MerR family transcriptional regulator | - |
| GLO19_RS10475 (2174289) | 2174289..2174870 | - | 582 | WP_002380883.1 | histidine phosphatase family protein | - |
| GLO19_RS10480 (2175092) | 2175092..2175421 | + | 330 | WP_002365236.1 | helix-turn-helix domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | - | - | 2108079..2176486 | 68407 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 218 a.a. Molecular weight: 26064.07 Da Isoelectric Point: 6.7559
>T143725 WP_002380879.1 NZ_CP046247:2170407-2171060 [Enterococcus faecalis]
MIGLLLVDFISKKLYFEAFNFSNELIKEVSYYSSKNIEQVKCFDIEKYVKEVEDVEFVEYTFQKQLKRRMLGSISKVDDE
VIITTNKELMLERKNFTKMHEVMHYYIDIPKINNATHTFSDILLKNGYLMEDFPKEYRANVGASMLMANDQALFYALKKF
YSFEEIAQYFFMSKSALRNRLIEHLMYVNNCTFAHANTLFNNYYFHKETDIYRFIFN
MIGLLLVDFISKKLYFEAFNFSNELIKEVSYYSSKNIEQVKCFDIEKYVKEVEDVEFVEYTFQKQLKRRMLGSISKVDDE
VIITTNKELMLERKNFTKMHEVMHYYIDIPKINNATHTFSDILLKNGYLMEDFPKEYRANVGASMLMANDQALFYALKKF
YSFEEIAQYFFMSKSALRNRLIEHLMYVNNCTFAHANTLFNNYYFHKETDIYRFIFN
Download Length: 654 bp
>T143725 NZ_CP061338:21771-22076 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCTTGCTGTCAGATAAAGTCTCCCGTGAACTTTATCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCTGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGGGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCTTGCTGTCAGATAAAGTCTCCCGTGAACTTTATCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCTGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGGGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA
Antitoxin
Download Length: 128 a.a. Molecular weight: 15031.09 Da Isoelectric Point: 5.8480
>AT143725 WP_002380877.1 NZ_CP046247:2170027-2170410 [Enterococcus faecalis]
VQPLAKRLQLLREEKEWTKTYVAKQLGLNNLGTYANWEYGTREPDSEMLSKIASLYDVSTDFLLGRTEERIHLNDKEKIG
KNIISHFRLNTSDMNIEDIEELEEELIDFQDFLIKKAKEKKKRNKKD
VQPLAKRLQLLREEKEWTKTYVAKQLGLNNLGTYANWEYGTREPDSEMLSKIASLYDVSTDFLLGRTEERIHLNDKEKIG
KNIISHFRLNTSDMNIEDIEELEEELIDFQDFLIKKAKEKKKRNKKD
Download Length: 384 bp
>AT143725 NZ_CP061338:21551-21769 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGCGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGCGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|