Detailed information of TA system TA143705

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 6649..7757
• Replicon: plasmid unnamed2
• Accession: NZ_CP046168
• Organism: Enterococcus faecium strain WGS1811-4-7

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: -
• Locus tag: F9Y88_RS15680
• Protein ID: WP_060763469.1
• Coordinates: 6649..7518 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: F9Y88_RS15685
• Protein ID: WP_000205227.1
• Coordinates: 7533..7757 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F9Y88_RS15655	2719..3291	-	573	WP_001079938.1	HTH domain-containing protein	-
F9Y88_RS15660	3567..4361	-	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
F9Y88_RS15665	4454..4996	-	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
F9Y88_RS15670	4993..5901	-	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
F9Y88_RS15675	5934..6668	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
F9Y88_RS15680	6649..7518	-	870	WP_060763469.1	nucleotidyltransferase domain-containing protein	Toxin
F9Y88_RS15685	7533..7757	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
F9Y88_RS15690	7900..9462	-	1563	WP_000136908.1	recombinase family protein	-
F9Y88_RS15695	9464..9880	-	417	WP_000323438.1	recombinase	-
F9Y88_RS15700	9881..10177	-	297	WP_002360685.1	hypothetical protein	-
F9Y88_RS15705	10689..11366	-	678	Protein_14	DNA topoisomerase	-
F9Y88_RS15710	11382..11984	-	603	WP_001062586.1	recombinase family protein	-
F9Y88_RS15715	11998..12168	-	171	WP_000713595.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-III / ant(6)-Ia / erm(B)	-	1..30099	30099

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32777.35 Da
• Isoelectric Point: 4.7269

>T143705 WP_060763469.1 NZ_CP046168:c7518-6649 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T143705 NZ_CP061335:c3910335-3909949 [Pseudomonas asiatica]
TTGCGTGTCAGCGGCCGCTGGCACCAGGCGGGCCGGCCAGTGGTATATGCCGCCACCAGCCCGGCCGGGGCAATGCTGGA
GGTGCTGGTGCACCTGGAGATCGACCCTGAGGATTTCCCCACCACCATGCGGCTGCTGCGTATCGAGTTGCCCGACACCG
TTTCGCAAGCGCAGTTGCCCGCCTTGCAGCCTGGCTGGTCGAGCCAGACCGAACTGACCCGGGGCCTGGGCAACCGTTTT
CTGGATGAGTGTTCGGCGTTGCTGCTGCCGGTGCCGAGCGCGATCATGCCCAGCACCACCAATTACCTGTTCAACCCGCG
CCATCCGCAGGCAAACAGCGCCAGGCTCGAGGTCGAGGACTTCACCCCGGACAGCCGGCTGTTTTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT143705 WP_000205227.1 NZ_CP046168:c7757-7533 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT143705 NZ_CP061335:c3910832-3910383 [Pseudomonas asiatica]
ATGCTTGCCGAAGTGCTTCGCGACAATGGTTACCACGAGTACCGGGCGCGCCTGCATGCACTGCTGGAAATCCCCGAACT
CGCCAGTGACTTCGAAATCCACACCCGTATCACCCATGGCTTCGCTGCCACCTGGCTGGTGAAACTGACCGAACTCGGCG
TGTTCACCCCTGTGGAGCGTGACCAGATCATTCCCCTGCGCACGCTGAAAACCCGCATCGAACGTGACCAGCCGCTGACC
GTGGAAGAAAGTGACCGGCTGTTCCGCTCGGCCCATATCACCGCCATGGCCGAAGCCGTGTTCGGTGAAGCGGGCAAGGC
AAAACGCTGGCTGTCCAAGCCCAAGGAGCGCTTCTCGGGGCTGACGCCGATGCAGATGCTCACCACCCAGCAGGGCACCA
CCCAGGTCGAGGAGATGTTGCTGCAGATCGCCGAGGGCTTCGGCCTGTGA