Detailed information of TA system TA143704

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 63..1200
• Replicon: plasmid unnamed2
• Accession: NZ_CP046168
• Organism: Enterococcus faecium strain WGS1811-4-7

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: F9Y88_RS15635
• Protein ID: WP_074394525.1
• Coordinates: 63..926 (-)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: Q9AL01
• Locus tag: F9Y88_RS15640
• Protein ID: WP_002331065.1
• Coordinates: 928..1200 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F9Y88_RS15635	63..926	-	864	WP_074394525.1	zeta toxin family protein	Toxin
F9Y88_RS15640	928..1200	-	273	WP_002331065.1	antitoxin	Antitoxin
F9Y88_RS15645	1217..1426	-	210	WP_000527318.1	peptide-binding protein	-
F9Y88_RS15650	1525..2421	-	897	WP_002334978.1	ParA family protein	-
F9Y88_RS15655	2719..3291	-	573	WP_001079938.1	HTH domain-containing protein	-
F9Y88_RS15660	3567..4361	-	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
F9Y88_RS15665	4454..4996	-	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
F9Y88_RS15670	4993..5901	-	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-III / ant(6)-Ia / erm(B)	-	1..30099	30099

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32611.17 Da
• Isoelectric Point: 6.6627

>T143704 WP_074394525.1 NZ_CP046168:c926-63 [Enterococcus faecium]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEALISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTPPIPKTPKLPGI

Nucleotide

• Length: 864 bp

>T143704 NZ_CP061335:2602611-2602892 [Pseudomonas asiatica]
ATGACCTATAAACTCGAATTCCTGCCTTCGGCACTGAAAGAGTGGGGCAAGCTAGGCCACACCGTGCGCGAACAGATCAA
GAAAAAACTCGCCGAGCGCCTGCAGTCACCCAAGGTACAGGCCGACGCGCTGCGTGACCTGCCGAACCACTACAAAATCA
AGCTCAAGGCCTCTGGATATCGCCTCGTCTATCGAGTCGAGGACGAGCGGATCGTTGTAGTGGTTGTATCGGTTGGCAAA
CGCGAGCGTAGCGAGGTGTATCGATCCGCTCAGAAGCGGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10733.17 Da
• Isoelectric Point: 4.4865

>AT143704 WP_002331065.1 NZ_CP046168:c1200-928 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILNKEKVA

Nucleotide

• Length: 273 bp

>AT143704 NZ_CP061335:2602376-2602621 [Pseudomonas asiatica]
ATGCAAAACATATTCGCTGACGTGGCCGTAAGCGTGTCGGAACTGAAAAAGAACCCATCCGCGGTGATGACCGGCGCTGC
GGGTATGCCCGTCGCCGTGCTCAATCACAACCGCGTGATGGGTTACATGGTGCCGGCCGAACTGTACGAGGCCATGATGG
AGCGCTTGGAAGATCTCGAATTGAGCGAACTGGCCAAGTCGAGGTTGGGTGAAAAAGGCGTACCGGTTTCCCTGGATGAC
CTATAA

Structures

Antitoxin

• AlphaFold DB: A0A828ZL55