Detailed information of TA system TA143703

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 47500..48107
• Replicon: plasmid unnamed1
• Accession: NZ_CP046167
• Organism: Enterococcus faecium strain WGS1811-4-7

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: F9Y88_RS14630
• Protein ID: WP_002321032.1
• Coordinates: 47757..48107 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: F9Y88_RS14625
• Protein ID: WP_002287514.1
• Coordinates: 47500..47763 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F9Y88_RS14585	42536..43435	+	900	WP_010733696.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
F9Y88_RS14590	43438..43935	+	498	WP_048946612.1	single-stranded DNA-binding protein	-
F9Y88_RS14595	44301..44561	+	261	WP_002296240.1	hypothetical protein	-
F9Y88_RS14600	45006..45212	+	207	WP_002324499.1	hypothetical protein	-
F9Y88_RS14605	45212..45463	+	252	WP_002287517.1	hypothetical protein	-
F9Y88_RS14610	45479..45916	+	438	WP_002332738.1	hypothetical protein	-
F9Y88_RS14615	45909..46616	+	708	WP_002332739.1	hypothetical protein	-
F9Y88_RS14620	46996..47175	+	180	WP_002305761.1	hypothetical protein	-
F9Y88_RS14625	47500..47763	+	264	WP_002287514.1	hypothetical protein	Antitoxin
F9Y88_RS14630	47757..48107	+	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
F9Y88_RS14635	48131..48745	-	615	WP_002318230.1	recombinase family protein	-
F9Y88_RS14640	49059..49481	+	423	WP_002288787.1	hypothetical protein	-
F9Y88_RS14645	49491..49694	+	204	WP_002299573.1	hypothetical protein	-
F9Y88_RS14650	49905..50489	+	585	WP_002299575.1	recombinase family protein	-
F9Y88_RS14655	50773..51453	-	681	WP_010861579.1	IS6-like element IS1216 family transposase	-
F9Y88_RS14660	51772..52644	-	873	WP_002305879.1	ROK family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..234807	234807

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T143703 WP_002321032.1 NZ_CP046167:47757-48107 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T143703 NZ_CP061335:1391302-1391598 [Pseudomonas asiatica]
ATGAGCGAAAAGTTGATCCCGTTTGATATGGCGGCCTTGTTGAGTAGTGATGAGGCCATCACTGAGTACCTTAGTCAGGT
CCTCGCAGATGGCGATACCGAGGAAATAATTCGGGCACTTGGCCATATCGCTCGCGCACGGGGAATGACGCGTATCGCCA
CCGAGAGTGGGTTGGGAAGGGAAAGTCTGTACAAGGCACTCAGTCCAGGCGCAAAACCACGCTTTGACACTGTGCTGAAA
GTCATTCGTGCCTTGGGTGTCGACCTCCTCGTTCAGCCCCATGTGGTTCCCCGCTAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT143703 WP_002287514.1 NZ_CP046167:47500-47763 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT143703 NZ_CP061335:1391012-1391305 [Pseudomonas asiatica]
ATGTATCTCGTTGAACAGACGGAGAGCTTTGCAGTTTGGCTATCCAGCTTGAGAGATTTGAGGGCCCAGCTTGCGATAGG
TCGTAGATTGGAGCGAGCAGCAATGGGGAATCTTGGAGATGTCAAATGGCTTGGAGGCGGTATCGGTGAGCTTCGCATCG
ATGTAGCTGCTGGATATCGTGTCTATTTTGCGCAAAAGGGCCAGAGGCTGGTTCTGCTGTTGGTCGGTGGAGACAAGTCC
ACCCAGGCCACCGATATCCTCAAAGCCAGAAAACTGCTGAAGGAGATGAAATGA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0