Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 496015..496586 Replicon chromosome
Accession NZ_CP046166
Organism Enterococcus faecium strain WGS1811-4-7

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag F9Y88_RS02385 Protein ID WP_002286801.1
Coordinates 496245..496586 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag F9Y88_RS02380 Protein ID WP_002323011.1
Coordinates 496015..496245 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F9Y88_RS02350 491148..492479 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
F9Y88_RS02355 492501..493127 + 627 WP_002286816.1 cysteine hydrolase -
F9Y88_RS02360 493310..493891 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
F9Y88_RS02365 494623..494970 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
F9Y88_RS02370 494946..495185 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
F9Y88_RS02375 495390..495728 - 339 WP_002286804.1 hypothetical protein -
F9Y88_RS02380 496015..496245 + 231 WP_002323011.1 hypothetical protein Antitoxin
F9Y88_RS02385 496245..496586 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
F9Y88_RS02390 497436..497621 + 186 WP_002304207.1 hypothetical protein -
F9Y88_RS02395 498126..498320 + 195 WP_002297028.1 hypothetical protein -
F9Y88_RS02400 498511..498760 + 250 Protein_476 transposase -
F9Y88_RS02405 499004..499834 - 831 WP_002286789.1 manganese catalase family protein -
F9Y88_RS02410 500042..501376 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T143702 WP_002286801.1 NZ_CP046166:496245-496586 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T143702 NZ_CP061335:1391012-1391305 [Pseudomonas asiatica]
ATGTATCTCGTTGAACAGACGGAGAGCTTTGCAGTTTGGCTATCCAGCTTGAGAGATTTGAGGGCCCAGCTTGCGATAGG
TCGTAGATTGGAGCGAGCAGCAATGGGGAATCTTGGAGATGTCAAATGGCTTGGAGGCGGTATCGGTGAGCTTCGCATCG
ATGTAGCTGCTGGATATCGTGTCTATTTTGCGCAAAAGGGCCAGAGGCTGGTTCTGCTGTTGGTCGGTGGAGACAAGTCC
ACCCAGGCCACCGATATCCTCAAAGCCAGAAAACTGCTGAAGGAGATGAAATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT143702 WP_002323011.1 NZ_CP046166:496015-496245 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT143702 NZ_CP061335:1391302-1391598 [Pseudomonas asiatica]
ATGAGCGAAAAGTTGATCCCGTTTGATATGGCGGCCTTGTTGAGTAGTGATGAGGCCATCACTGAGTACCTTAGTCAGGT
CCTCGCAGATGGCGATACCGAGGAAATAATTCGGGCACTTGGCCATATCGCTCGCGCACGGGGAATGACGCGTATCGCCA
CCGAGAGTGGGTTGGGAAGGGAAAGTCTGTACAAGGCACTCAGTCCAGGCGCAAAACCACGCTTTGACACTGTGCTGAAA
GTCATTCGTGCCTTGGGTGTCGACCTCCTCGTTCAGCCCCATGTGGTTCCCCGCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References