Detailed information of TA system TA143699

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 370802..371631
• Replicon: plasmid pTHAF191c_c
• Accession: NZ_CP046164
• Organism: Vibrio sp. THAF191c

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GGC03_RS26415
• Protein ID: WP_152472731.1
• Coordinates: 370802..371365 (-)
• Length: 188 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7Y3YYU7
• Locus tag: GGC03_RS26420
• Protein ID: WP_040122444.1
• Coordinates: 371362..371631 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GGC03_RS26400	365864..366832	-	969	WP_152472734.1	hypothetical protein	-
GGC03_RS26405	366964..367236	-	273	WP_152472733.1	hypothetical protein	-
GGC03_RS26410	367301..369394	-	2094	WP_152472732.1	TonB-dependent siderophore receptor	-
GGC03_RS26415	370802..371365	-	564	WP_152472731.1	GNAT family N-acetyltransferase	Toxin
GGC03_RS26420	371362..371631	-	270	WP_040122444.1	DUF1778 domain-containing protein	Antitoxin
GGC03_RS26425	371838..372260	-	423	WP_152472730.1	GNAT family N-acetyltransferase	-
GGC03_RS26430	372388..372792	-	405	WP_152472729.1	NUDIX domain-containing protein	-
GGC03_RS26435	373239..373715	-	477	WP_152472924.1	DUF1311 domain-containing protein	-
GGC03_RS26440	373882..374379	-	498	WP_152472728.1	GNAT family N-acetyltransferase	-
GGC03_RS26445	374441..374809	-	369	WP_152472727.1	hypothetical protein	-
GGC03_RS26450	374939..375316	-	378	WP_152472726.1	hypothetical protein	-
GGC03_RS26455	375452..375949	-	498	WP_152472725.1	DUF2867 domain-containing protein	-
GGC03_RS26460	376049..376381	-	333	WP_040122455.1	YnfA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..396310	396310

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-81)

Sequences

Toxin

Protein

• Length: 188 a.a.
• Molecular weight: 21099.61 Da
• Isoelectric Point: 9.3822

>T143699 WP_152472731.1 NZ_CP046164:c371365-370802 [Vibrio sp. THAF191c]
VSWGNEFVELSKSMHDRSSFDCGESELNTFIKTLAAKHMQAGISRTMVLPSSQPLLNQKYAICAFYSVAPSSISRETLPA
KLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKISLIRALKYLWEVNHHMRAYAIIVDCLTDSAQAFYTKFGFEVLCEHNG
RIRMFLPMKTVEQLFSLKATNITKDDV

Nucleotide

• Length: 564 bp

>T143699 NZ_CP061329:c4350060-4349887 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9839.34 Da
• Isoelectric Point: 4.5482

>AT143699 WP_040122444.1 NZ_CP046164:c371631-371362 [Vibrio sp. THAF191c]
MATARLDIRLDEEIKAKAEKASALLGLKSLTEYVVRLMDEDATQVIEQHESIVVKESVFDEFMMACDKAKAPNQALLDAA
KLTNESGIK

Nucleotide

• Length: 270 bp

>AT143699 NZ_CP061329:c4350384-4350088 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A7Y3YYU7