Detailed information of TA system TA143697

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 165914..166442
• Replicon: plasmid pTHAF191c_c
• Accession: NZ_CP046164
• Organism: Vibrio sp. THAF191c

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GGC03_RS25385
• Protein ID: WP_152472625.1
• Coordinates: 165914..166204 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A7Y3YX34
• Locus tag: GGC03_RS25390
• Protein ID: WP_006958739.1
• Coordinates: 166194..166442 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GGC03_RS25350	161423..162166	+	744	WP_152472629.1	transporter substrate-binding domain-containing protein	-
GGC03_RS25355	162617..163072	-	456	WP_152472628.1	DUF2079 domain-containing protein	-
GGC03_RS25360	163069..164235	-	1167	WP_152472906.1	hypothetical protein	-
GGC03_RS25365	164525..164638	+	114	WP_152472627.1	DUF3265 domain-containing protein	-
GGC03_RS25370	164694..164993	-	300	WP_043011872.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GGC03_RS25375	165001..165243	-	243	WP_152472626.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GGC03_RS25380	165543..165755	+	213	WP_173020392.1	hypothetical protein	-
GGC03_RS25385	165914..166204	-	291	WP_152472625.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GGC03_RS25390	166194..166442	-	249	WP_006958739.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GGC03_RS25395	166499..166627	+	129	WP_144382900.1	DUF3265 domain-containing protein	-
GGC03_RS25400	166773..167363	+	591	WP_040122585.1	Crp/Fnr family transcriptional regulator	-
GGC03_RS25405	167425..167871	+	447	WP_152472624.1	isoprenylcysteine carboxylmethyltransferase family protein	-
GGC03_RS25410	167993..168220	+	228	Protein_150	isocitrate lyase/phosphoenolpyruvate mutase family protein	-
GGC03_RS25415	168622..169101	-	480	WP_152472623.1	hypothetical protein	-
GGC03_RS25420	169882..170217	+	336	WP_064487238.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..396310	396310

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (12-75)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11247.22 Da
• Isoelectric Point: 10.5339

>T143697 WP_152472625.1 NZ_CP046164:c166204-165914 [Vibrio sp. THAF191c]
MTYKLDFKKSALKEWKKLGATLQQQFKKKLIERLDNPHVLASKLSGADNMYKIKLRQSGYRLVYKVEDDVIIVTVLAVGK
RERSDVYRKAMNRLDD

Nucleotide

• Length: 291 bp

>T143697 NZ_CP061329:3543611-3543829 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9030.29 Da
• Isoelectric Point: 3.9610

>AT143697 WP_006958739.1 NZ_CP046164:c166442-166194 [Vibrio sp. THAF191c]
MTTRILADVAASITELKANPMKVATSANGEPVAVLNRNEPAFYCVPAEAYEMMMDRLEDLELLAIAKERESEESISVNID
DL

Nucleotide

• Length: 249 bp

>AT143697 NZ_CP061329:3543211-3543585 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A7Y3YX34