Detailed information of TA system TA143696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-YefM
• Location: 164694..165243
• Replicon: plasmid pTHAF191c_c
• Accession: NZ_CP046164
• Organism: Vibrio sp. THAF191c

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A2A2MR35
• Locus tag: GGC03_RS25370
• Protein ID: WP_043011872.1
• Coordinates: 164694..164993 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: GGC03_RS25375
• Protein ID: WP_152472626.1
• Coordinates: 165001..165243 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GGC03_RS25345	160370..161254	+	885	WP_152472630.1	LysR family transcriptional regulator	-
GGC03_RS25350	161423..162166	+	744	WP_152472629.1	transporter substrate-binding domain-containing protein	-
GGC03_RS25355	162617..163072	-	456	WP_152472628.1	DUF2079 domain-containing protein	-
GGC03_RS25360	163069..164235	-	1167	WP_152472906.1	hypothetical protein	-
GGC03_RS25365	164525..164638	+	114	WP_152472627.1	DUF3265 domain-containing protein	-
GGC03_RS25370	164694..164993	-	300	WP_043011872.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GGC03_RS25375	165001..165243	-	243	WP_152472626.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GGC03_RS25380	165543..165755	+	213	WP_173020392.1	hypothetical protein	-
GGC03_RS25385	165914..166204	-	291	WP_152472625.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GGC03_RS25390	166194..166442	-	249	WP_006958739.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GGC03_RS25395	166499..166627	+	129	WP_144382900.1	DUF3265 domain-containing protein	-
GGC03_RS25400	166773..167363	+	591	WP_040122585.1	Crp/Fnr family transcriptional regulator	-
GGC03_RS25405	167425..167871	+	447	WP_152472624.1	isoprenylcysteine carboxylmethyltransferase family protein	-
GGC03_RS25410	167993..168220	+	228	Protein_150	isocitrate lyase/phosphoenolpyruvate mutase family protein	-
GGC03_RS25415	168622..169101	-	480	WP_152472623.1	hypothetical protein	-
GGC03_RS25420	169882..170217	+	336	WP_064487238.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..396310	396310

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-93)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-55)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11439.05 Da
• Isoelectric Point: 9.3232

>T143696 WP_043011872.1 NZ_CP046164:c164993-164694 [Vibrio sp. THAF191c]
MQKNKYKLSNLAQSHLHKIKNYTVENFSELQWRNYKDTLLSGFQMLADNPGLGRSCDEIYPNGFYFPIGKHTAYFTKEDG
FILVVAVLGQSQLPQNHLK

Nucleotide

• Length: 300 bp

>T143696 NZ_CP061329:2723747-2723854 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9073.29 Da
• Isoelectric Point: 5.1051

>AT143696 WP_152472626.1 NZ_CP046164:c165243-165001 [Vibrio sp. THAF191c]
MYTLTANDAKRNFGELLLSAQREPVKISKNSKDTVVVMSIRDFEELEAMKVEYLKHCFKSAKEDLNNGNTVDGESFLNSL

Nucleotide

• Length: 243 bp

>AT143696 NZ_CP061329:c2723697-2723634 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A2A2MR35