Detailed information of TA system TA143695

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 3253..4124
• Replicon: plasmid unnamed4
• Accession: NZ_CP046159
• Organism: Leptolyngbya sp. BL0902

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: -
• Locus tag: GFS31_RS20585
• Protein ID: WP_198808633.1
• Coordinates: 3681..4124 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: -
• Locus tag: GFS31_RS20580
• Protein ID: WP_198808632.1
• Coordinates: 3253..3684 (+)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFS31_RS20570	107..892	+	786	WP_198808630.1	type I-D CRISPR-associated protein Cas5/Csc1	-
GFS31_RS20575	885..3215	+	2331	WP_198808631.1	type I-D CRISPR-associated helicase Cas3'	-
GFS31_RS20580	3253..3684	+	432	WP_198808632.1	nucleotidyltransferase domain-containing protein	Antitoxin
GFS31_RS20585	3681..4124	+	444	WP_198808633.1	DUF86 domain-containing protein	Toxin
GFS31_RS20590	4139..5020	+	882	WP_198808634.1	CRISPR-associated endoribonuclease Cas6	-
GFS31_RS20595	5087..5680	+	594	WP_198808635.1	CRISPR-associated protein Cas4	-
GFS31_RS20600	5706..6707	+	1002	WP_198808636.1	type I-D CRISPR-associated endonuclease Cas1	-
GFS31_RS20605	6803..7075	+	273	WP_198808637.1	CRISPR-associated endonuclease Cas2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..44466	44466

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (20-137)

Antitoxin

• Polymerase beta, nucleotidyltransferase (13-105)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16575.86 Da
• Isoelectric Point: 4.5070

>T143695 WP_198808633.1 NZ_CP046159:3681-4124 [Leptolyngbya sp. BL0902]
MSQLNREAILSKLSRMVDRLDSLKRFEAISLSAYLEDEDCQLIVERQLELLIQSALDINKAFLKRATSPKNSAAISNYES
FMVMGELGFLPPTLAQAIAPSGSFRNVLAHDYDDIDADQVYAALQKALTQYPQYVRAIHTYLDTLED

Nucleotide

• Length: 444 bp

>T143695 NZ_CP061329:2723212-2723319 [Escherichia coli]
ATGACGCTCGCGAAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 16206.45 Da
• Isoelectric Point: 6.6387

>AT143695 WP_198808632.1 NZ_CP046159:3253-3684 [Leptolyngbya sp. BL0902]
MTDFAKIIALSSQIADHYPTLKLLILFGSRARGDHAPSSDWDMAYLCEPSPAKGEPWFPRADLLLTLSEHSHISSNRLDL
IDLSTCSDILAHVVAQEGKLVYEREPGEFERFRQQALKAPAELKQYRQTQREKVLQALARWGS

Nucleotide

• Length: 432 bp

>AT143695 NZ_CP061329:c2723159-2723098 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT