Detailed information of TA system TA143688

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 457090..457740
• Replicon: chromosome
• Accession: NZ_CP046155
• Organism: Leptolyngbya sp. BL0902

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GFS31_RS02090
• Protein ID: WP_198806655.1
• Coordinates: 457339..457740 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GFS31_RS02085
• Protein ID: WP_198806654.1
• Coordinates: 457090..457329 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFS31_RS02050	453248..453463	+	216	WP_198806647.1	hypothetical protein	-
GFS31_RS02055	453483..453989	+	507	WP_198806648.1	hypothetical protein	-
GFS31_RS02060	454276..455223	+	948	WP_198806649.1	hypothetical protein	-
GFS31_RS02065	455525..456010	+	486	WP_198806650.1	PIN domain-containing protein	-
GFS31_RS02070	455997..456203	+	207	WP_198806651.1	hypothetical protein	-
GFS31_RS02075	456297..456524	+	228	WP_198806652.1	hypothetical protein	-
GFS31_RS02080	456514..456903	+	390	WP_198806653.1	type II toxin-antitoxin system VapC family toxin	-
GFS31_RS02085	457090..457329	+	240	WP_198806654.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GFS31_RS02090	457339..457740	+	402	WP_198806655.1	type II toxin-antitoxin system VapC family toxin	Toxin
GFS31_RS02095	457855..458274	+	420	WP_198806656.1	XisH family protein	-
GFS31_RS02100	458259..458570	+	312	WP_198806657.1	XisI protein	-
GFS31_RS02105	458734..459033	+	300	WP_198806658.1	DUF2281 domain-containing protein	-
GFS31_RS02110	459020..459379	+	360	WP_198806659.1	type II toxin-antitoxin system PemK/MazF family toxin	-
GFS31_RS02115	459542..459754	+	213	WP_198806660.1	Uma2 family endonuclease	-
GFS31_RS02120	459753..459965	+	213	Protein_418	IS1 family transposase	-
GFS31_RS02125	460068..460973	-	906	WP_198807988.1	phosphodiester glycosidase family protein	-
GFS31_RS02130	461182..461598	+	417	WP_198806661.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15060.37 Da
• Isoelectric Point: 5.4767

>T143688 WP_198806655.1 NZ_CP046155:457339-457740 [Leptolyngbya sp. BL0902]
MTYQYLLDTNILSDLVRHPQGQVFQQITKVGEESVCTSIVVACELRFGAVKSSSSRLIQQVERILDVLPILPLTTPIDHH
YAEIRHHLEQLGIPIGPNDLLIAAHTLSLELTLITANTREFERIPNLQIDNWL

Nucleotide

• Length: 402 bp

>T143688 NZ_CP061329:1300755-1301153 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8574.76 Da
• Isoelectric Point: 3.7605

>AT143688 WP_198806654.1 NZ_CP046155:457090-457329 [Leptolyngbya sp. BL0902]
MSNPNHAFLVASGQNQVLTIPSEFTLPSSEVLLRKEGNRLIIEPIQPGSLLALLSTLEDIADPFPDTDAETLSLDDIML

Nucleotide

• Length: 240 bp

>AT143688 NZ_CP061329:1300528-1300755 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA