Detailed information of TA system TA143682

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1228958..1229875
• Replicon: chromosome
• Accession: NZ_CP046145
• Organism: Bacillus velezensis strain PEBA20

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: GKO36_RS06355
• Protein ID: WP_007407256.1
• Coordinates: 1229129..1229875 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2C3Z8
• Locus tag: GKO36_RS06350
• Protein ID: WP_014417527.1
• Coordinates: 1228958..1229128 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKO36_RS06305	1224164..1224676	+	513	WP_012117362.1	sigma-70 family RNA polymerase sigma factor	-
GKO36_RS06310	1224789..1225448	+	660	WP_014417523.1	terminase	-
GKO36_RS06315	1225346..1225813	+	468	Protein_1182	hypothetical protein	-
GKO36_RS06320	1225826..1226197	+	372	WP_014417525.1	hypothetical protein	-
GKO36_RS06325	1226202..1226399	+	198	WP_007610833.1	XkdX family protein	-
GKO36_RS06330	1226456..1227217	+	762	WP_014417526.1	hypothetical protein	-
GKO36_RS06335	1227269..1227532	+	264	WP_003154815.1	hemolysin XhlA family protein	-
GKO36_RS06340	1227546..1227809	+	264	WP_003154813.1	phage holin	-
GKO36_RS06345	1227823..1228701	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
GKO36_RS06350	1228958..1229128	-	171	WP_014417527.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
GKO36_RS06355	1229129..1229875	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
GKO36_RS06360	1229979..1230977	-	999	WP_003154805.1	inorganic phosphate transporter	-
GKO36_RS06365	1230990..1231607	-	618	WP_003154804.1	DUF47 domain-containing protein	-
GKO36_RS06370	1231893..1233209	-	1317	WP_007610842.1	amino acid permease	-
GKO36_RS06375	1233531..1234481	+	951	WP_014417529.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T143682 WP_007407256.1 NZ_CP046145:c1229875-1229129 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T143682 NZ_CP061319:1526539-1526943 [Polynucleobacter sp. AM-7D1]
ATGAACGCTAAGAAAATATTTTTTGATACAAATACCCTGCTTTACCTGCTGTCATCGGATAACAAAAAAGCGGATTGGGT
AAGCAAAAATCTACAACAGCACAATGTGATTAGCGTTCAAGTGCTAAATGAATTTACTTCGGCCAGCCTCAGAAAAATTA
AAATATCCAACACTGAGCTAGATGAGTTTTTAGATTTATTTACATCGACATTTCAGGTGAGATCTCTAGATATGGATACC
TTCGAGACCGGACTAATGGTTTCTAGACGATATGGGTATCAACACTATGATTCAATGATTATTGCTGCAGCATTGCAAGC
TGGTTGCGAAAGGCTCTACTCAGAGGATATGCAGCACCGACAGGTAATCGATAAAAGACTACAAATCGTGAACCCTTTTG
TGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.78 Da
• Isoelectric Point: 10.9951

>AT143682 WP_014417527.1 NZ_CP046145:c1229128-1228958 [Bacillus velezensis]
MEQAFQNGQPEKRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT143682 NZ_CP061319:1526319-1526549 [Polynucleobacter sp. AM-7D1]
ATGATCGTTTCCAAATGGGGCAATAGCCTAGCTATTCGACTGCCTTCCCAAGTGGTGGATTATTTGAAACTTAAAGAAGG
TGATGACATTGAGGTTCATGTCACCGATAAAAAGCATTTTCAAATTAAGAAGAAACCATCTCTGCAAGAGAGGATTGAGG
GTCTGAGAAAATTCCGTGGGCGTATGCCTGCTGATTTTCACTTTGATAGATCCGAACTGAATGAACGCTAA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2C3Z8