Detailed information of TA system TA143681

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 478974..479611
• Replicon: chromosome
• Accession: NZ_CP046145
• Organism: Bacillus velezensis strain PEBA20

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: GKO36_RS02400
• Protein ID: WP_003156187.1
• Coordinates: 479261..479611 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: GKO36_RS02395
• Protein ID: WP_003156188.1
• Coordinates: 478974..479255 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKO36_RS02375	475339..475938	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
GKO36_RS02380	476031..476396	+	366	WP_014304402.1	holo-ACP synthase	-
GKO36_RS02385	476561..477568	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
GKO36_RS02390	477685..478854	+	1170	WP_014416966.1	alanine racemase	-
GKO36_RS02395	478974..479255	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
GKO36_RS02400	479261..479611	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
GKO36_RS02405	479729..480550	+	822	WP_003156182.1	STAS domain-containing protein	-
GKO36_RS02410	480555..480920	+	366	WP_007609584.1	RsbT antagonist protein RsbS	-
GKO36_RS02415	480923..481324	+	402	WP_003156178.1	anti-sigma regulatory factor	-
GKO36_RS02420	481336..482343	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
GKO36_RS02425	482407..482736	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
GKO36_RS02430	482733..483215	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
GKO36_RS02435	483181..483969	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
GKO36_RS02440	483969..484571	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T143681 WP_003156187.1 NZ_CP046145:479261-479611 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T143681 NZ_CP061319:651055-651378 [Polynucleobacter sp. AM-7D1]
ATGACTTATACCATTCGCTATTACAGCCCCGATGTATTGGAGCAAATTTTTGAATTACCTATTTCTTTGCAGGCGCGCTA
CATTGCTTTGACGCAGCGCATGATTGAATATGGGCCGCATTTAGGTTTGCCGCATACCGATTCGTTTGGCGGTGGTTTAT
TTGAGCTCAGGCTAAAAGGTATAGAGGGGATTGCAAGAGTTTTCTTTTGTACTGTGGTGGAGCGAGAGATTGTGATGTTG
CATAGCTTTATTAAGAAATCGCAGAAAACTCCCGATAAAGAACTAAGGCTAGCCAAGTTGAGAATGAGGGAAGTGAAATT
ATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT143681 WP_003156188.1 NZ_CP046145:478974-479255 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT143681 NZ_CP061319:651375-651674 [Polynucleobacter sp. AM-7D1]
ATGAAGACAAAGACATTGACCCATAAGCAAGCAGTTAGCAAGATGTTGAAAAATCCAGCAGTCAAGGCTGAGGTTGAGCG
CTTAAATAAAGAGGAGTTTGCGATTCTTGATGAAATACTGCAGGCTCGTAAAGCCGCAGGTTTATCTCAGGCTGAAGTGG
CTCGCAGAATGGGTACACAAGCTCCTGCAATAGCCAGACTCGAAAGCTCTTTGGCAACTGGAAAGCACTCCCCAAGTTTA
AGTACACTGCGAAAGTATGCTGCTGCCTTAGGTAAGAGGGTCGAATTACACCTAGTCTGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5