Detailed information of TA system TA143667

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4952543..4953159
• Replicon: chromosome
• Accession: NZ_CP046116
• Organism: Enterobacter cloacae strain CBG15936

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GJ694_RS23525
• Protein ID: WP_080292722.1
• Coordinates: 4952543..4952914 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8B3JKE9
• Locus tag: GJ694_RS23530
• Protein ID: WP_020686881.1
• Coordinates: 4952917..4953159 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ694_RS23510	4950043..4950945	+	903	WP_014833793.1	formate dehydrogenase subunit beta	-
GJ694_RS23515	4950942..4951577	+	636	WP_013099357.1	formate dehydrogenase cytochrome b556 subunit	-
GJ694_RS23520	4951574..4952503	+	930	WP_029882351.1	formate dehydrogenase accessory protein FdhE	-
GJ694_RS23525	4952543..4952914	-	372	WP_080292722.1	type II toxin-antitoxin system VapC family toxin	Toxin
GJ694_RS23530	4952917..4953159	-	243	WP_020686881.1	ribbon-helix-helix domain-containing protein	Antitoxin
GJ694_RS23535	4953359..4954276	+	918	WP_062935377.1	alpha/beta hydrolase	-
GJ694_RS23540	4954289..4955230	-	942	WP_013099362.1	fatty acid biosynthesis protein FabY	-
GJ694_RS23545	4955275..4955712	-	438	WP_013099363.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GJ694_RS23550	4955709..4956590	-	882	WP_013099364.1	virulence factor BrkB family protein	-
GJ694_RS23555	4956584..4957183	-	600	WP_013099365.1	glucose-1-phosphatase	-
GJ694_RS23560	4957303..4958103	-	801	WP_020686879.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13657.75 Da
• Isoelectric Point: 6.6407

>T143667 WP_080292722.1 NZ_CP046116:c4952914-4952543 [Enterobacter cloacae]
MNNMAVFDTNILIDLFNNRVEAADAIDHAASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFSGIDEVVTPYQL

Nucleotide

• Length: 372 bp

>T143667 NZ_CP061315:275943-276245 [Polynucleobacter sp. AP-Kolm-20A-A1]
ATGAAGAGTGTTAAAAAGACCAAAGTAAAAGATTCAAAAAAATTCGATGTTGTTGACTACCTGAAGACTGAAAAAGATAT
TGCCGCTTATCTTTCTGCTGTTTTAGAAGATGGCGATCCTGCATTGTTTGTTGCCGCCATTGGCGATATTGCCAGAGCTA
AAGGAATGACAGAGATTGCCAAGAAGAGTGGTGTTACGCGGGAGTCTTTGTATAGAGCTTTAAAAATAGAAGCTAGACCA
CGATTTGAAACTGTGACACGGGTGGTGCAGGCGCTAGGCATGAAACTGAGCGTTCACGCTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9369.68 Da
• Isoelectric Point: 4.4076

>AT143667 WP_020686881.1 NZ_CP046116:c4953159-4952917 [Enterobacter cloacae]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRAALGLWGDKKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT143667 NZ_CP061315:275646-275942 [Polynucleobacter sp. AP-Kolm-20A-A1]
ATGTATGAAATCAAAAAAACAGATGAATTTGAAAATTGGTTTTCCGGTATTCGCGACCCATTAACGCGAGGTCGACTCCT
AGCAAGACTGCGTAAGGCTTCATTAGGGAGTCTTGGGGATATAGCTTCAGTCGGTGATGGGGTGTGGGAGATGAGAGAGC
ATTTCGGTGCCGGCTACAGAATGTATTACGTTAGCTATCGAAGGACCATCATTCTGATGTTGGGTGGTGGATGCAAATCT
ACACAAATTGCTGATATTAAAAAGGTTAAGAAGTTGCTTCTAACCCTGGAGGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A8B3JKE9