Detailed information of TA system TA143666

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 4069993..4070653
• Replicon: chromosome
• Accession: NZ_CP046116
• Organism: Enterobacter cloacae strain CBG15936

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: A0A8H9S912
• Locus tag: GJ694_RS19320
• Protein ID: WP_020687087.1
• Coordinates: 4069993..4070406 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A5E1AG01
• Locus tag: GJ694_RS19325
• Protein ID: WP_013098615.1
• Coordinates: 4070387..4070653 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ694_RS19300	4065986..4067719	-	1734	WP_013098610.1	single-stranded-DNA-specific exonuclease RecJ	-
GJ694_RS19305	4067725..4068438	-	714	WP_013098611.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
GJ694_RS19310	4068467..4069363	-	897	WP_013098612.1	site-specific tyrosine recombinase XerD	-
GJ694_RS19315	4069465..4069986	+	522	WP_013098613.1	flavodoxin FldB	-
GJ694_RS19320	4069993..4070406	-	414	WP_020687087.1	protein YgfX	Toxin
GJ694_RS19325	4070387..4070653	-	267	WP_013098615.1	FAD assembly factor SdhE	Antitoxin
GJ694_RS19330	4070948..4071928	+	981	WP_129253273.1	tRNA-modifying protein YgfZ	-
GJ694_RS19335	4072038..4072697	-	660	WP_048970648.1	hemolysin III family protein	-
GJ694_RS19340	4072963..4073694	+	732	WP_020687085.1	MurR/RpiR family transcriptional regulator	-
GJ694_RS19345	4073811..4075244	+	1434	WP_038983905.1	6-phospho-beta-glucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 16220.17 Da
• Isoelectric Point: 10.7596

>T143666 WP_020687087.1 NZ_CP046116:c4070406-4069993 [Enterobacter cloacae]
VVLWQSDLRVSWRSQWMSLLLHGLVAAFVLLMPWPLSYTPLWLLLLSFVVFDSVRSQRRINARQGEIKLLMDSRLRWQGQ
EWDILGMPWMLSSGMMLRLRQVGGGKCQHLWLAADSMDIAEWRELRRMLLQQQPTQE

Nucleotide

• Length: 414 bp

>T143666 NZ_CP061315:275646-275942 [Polynucleobacter sp. AP-Kolm-20A-A1]
ATGTATGAAATCAAAAAAACAGATGAATTTGAAAATTGGTTTTCCGGTATTCGCGACCCATTAACGCGAGGTCGACTCCT
AGCAAGACTGCGTAAGGCTTCATTAGGGAGTCTTGGGGATATAGCTTCAGTCGGTGATGGGGTGTGGGAGATGAGAGAGC
ATTTCGGTGCCGGCTACAGAATGTATTACGTTAGCTATCGAAGGACCATCATTCTGATGTTGGGTGGTGGATGCAAATCT
ACACAAATTGCTGATATTAAAAAGGTTAAGAAGTTGCTTCTAACCCTGGAGGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10569.05 Da
• Isoelectric Point: 5.1456

>AT143666 WP_013098615.1 NZ_CP046116:c4070653-4070387 [Enterobacter cloacae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDSLSDEDKRLFVRLLESDDPDLFNWLMNHGKPADTELQRMVQLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT143666 NZ_CP061315:275943-276245 [Polynucleobacter sp. AP-Kolm-20A-A1]
ATGAAGAGTGTTAAAAAGACCAAAGTAAAAGATTCAAAAAAATTCGATGTTGTTGACTACCTGAAGACTGAAAAAGATAT
TGCCGCTTATCTTTCTGCTGTTTTAGAAGATGGCGATCCTGCATTGTTTGTTGCCGCCATTGGCGATATTGCCAGAGCTA
AAGGAATGACAGAGATTGCCAAGAAGAGTGGTGTTACGCGGGAGTCTTTGTATAGAGCTTTAAAAATAGAAGCTAGACCA
CGATTTGAAACTGTGACACGGGTGGTGCAGGCGCTAGGCATGAAACTGAGCGTTCACGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A5E1AG01