Detailed information of TA system TA143664

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 3229711..3230301
• Replicon: chromosome
• Accession: NZ_CP046116
• Organism: Enterobacter cloacae strain CBG15936

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: GJ694_RS15435
• Protein ID: WP_129252264.1
• Coordinates: 3229711..3230043 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: GJ694_RS15440
• Protein ID: WP_129252261.1
• Coordinates: 3230044..3230301 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ694_RS15420	3225870..3227195	+	1326	WP_129252266.1	SidA/IucD/PvdA family monooxygenase	-
GJ694_RS15425	3227222..3229411	+	2190	WP_013097826.1	TonB-dependent siderophore receptor	-
GJ694_RS15435	3229711..3230043	-	333	WP_129252264.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GJ694_RS15440	3230044..3230301	-	258	WP_129252261.1	antitoxin	Antitoxin
GJ694_RS15445	3230912..3231610	+	699	Protein_3032	hypothetical protein	-
GJ694_RS15450	3231626..3232323	-	698	Protein_3033	IS1 family transposase	-
GJ694_RS15455	3232398..3233201	+	804	Protein_3034	hypothetical protein	-
GJ694_RS15460	3233581..3234540	-	960	WP_038979636.1	zinc-binding alcohol dehydrogenase family protein	-
GJ694_RS15465	3234588..3234977	-	390	WP_013097827.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3231626..3232003	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-85)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11807.71 Da
• Isoelectric Point: 10.5834

>T143664 WP_129252264.1 NZ_CP046116:c3230043-3229711 [Enterobacter cloacae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKVTRLPVVVPVTSGGNFARTAGFTVSLDGAGTKTMGIIRCDQPRTI
DIVARNGKRLERVPDAVVNEVLARLDAILS

Nucleotide

• Length: 333 bp

>T143664 NZ_CP061314:c1205102-1204893 [Polynucleobacter corsicus]
TTGGTTTGGCTGATTGAGTTTGCCCCATTAGCAGAAAAACAATTAAGAAAATTTGATCCTAAAGTAGCTAAAAGAATTTT
TAAGTTTTGCAGAGAGAGGCTTGCCGTTCAGGCTGACCCAAGAATTCTTGGCGAAGCTTTAAAAGGTAGCGAGTATGGTG
ACTACTGGCGGTATCGGGTCGGCGATTATCGAATTATTGCTTTTATTTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9437.85 Da
• Isoelectric Point: 4.2333

>AT143664 WP_129252261.1 NZ_CP046116:c3230301-3230044 [Enterobacter cloacae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSIDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAT
GQEIL

Nucleotide

• Length: 258 bp

>AT143664 NZ_CP061314:c1205310-1205086 [Polynucleobacter corsicus]
ATGCTAGCAATACGATTACCCAAGGAAATTGAATTGAGACTTGATAATTTAGCCAAGGCAACTGGCCGAACTAAAACCTT
TTATGCTCGTGAAGCGATTCTGGCTTATGTGGAGGATATGGAAGACTTATATCTAGCTGATAAGAGTATGAGAGCATTAA
AAAGTGGCAAAGTAAAAGCTATCCCATTGGACCAAGTGGAGCGAAATCTTGGTTTGGCTGATTGA