Detailed information of TA system TA143654

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1144043..1144663
• Replicon: chromosome
• Accession: NZ_CP046116
• Organism: Enterobacter cloacae strain CBG15936

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A5E1A1U8
• Locus tag: GJ694_RS05225
• Protein ID: WP_013095889.1
• Coordinates: 1144043..1144261 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: GJ694_RS05230
• Protein ID: WP_129252903.1
• Coordinates: 1144289..1144663 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ694_RS05195	1140055..1140315	+	261	WP_006176933.1	type B 50S ribosomal protein L31	-
GJ694_RS05200	1140318..1140458	+	141	WP_003859006.1	type B 50S ribosomal protein L36	-
GJ694_RS05205	1140455..1141165	-	711	WP_129252901.1	GNAT family N-acetyltransferase	-
GJ694_RS05210	1141267..1142727	+	1461	WP_048970693.1	PLP-dependent aminotransferase family protein	-
GJ694_RS05215	1142699..1143166	-	468	WP_013095887.1	membrane protein	-
GJ694_RS05220	1143282..1143833	-	552	WP_013095888.1	maltose O-acetyltransferase	-
GJ694_RS05225	1144043..1144261	-	219	WP_013095889.1	hemolysin expression modulator Hha	Toxin
GJ694_RS05230	1144289..1144663	-	375	WP_129252903.1	Hha toxicity modulator TomB	Antitoxin
GJ694_RS05235	1145176..1148322	-	3147	WP_013095890.1	multidrug efflux RND transporter permease subunit	-
GJ694_RS05240	1148345..1149538	-	1194	WP_023620093.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8625.98 Da
• Isoelectric Point: 8.9008

>T143654 WP_013095889.1 NZ_CP046116:c1144261-1144043 [Enterobacter cloacae]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELTVFYSAADHRLAELTMNKLYDKIPPSVWKFVR

Nucleotide

• Length: 219 bp

>T143654 NZ_CP061306:1165949-1166239 [Polynucleobacter sp. JS-JIR-II-b4]
ATGCATGAAATAAGGAAAACAGAAGAATTTAAGTCCTGGTTAGATTCCCTTAATGATTTGATGGGGAGGGCAAAAATTCA
AGCCAGAATTAAACGTTTGGCTGAAGGCAATTCAGGAAATACAAAATCCGTTGGCAGCAAAGTCTTTGAGATGAAGATTG
ATTTTGGACCCGGCTATAGGATTTATTACACAAAACACCAGAGCGCGATATATCTATTACTTATCGGTGGCGATAAAAGA
ACTCAGAGCAAAGATATTCAGATAGCAAAATATTTGGCAAAACACATATAG

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14455.24 Da
• Isoelectric Point: 5.1481

>AT143654 WP_129252903.1 NZ_CP046116:c1144663-1144289 [Enterobacter cloacae]
MDEYSPKRHDIAQLKFLCESLHHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Nucleotide

• Length: 375 bp

>AT143654 NZ_CP061306:1166248-1166556 [Polynucleobacter sp. JS-JIR-II-b4]
ATGAAAAAAACAGTTACCAGCCCTTATGATGTAGCAGAACATTTGCGCACCCCCAAAGAAATGTCTCTTTATCTACAGGC
GTGTATTGAGGAATCCAACGGAGACGCAGCGTTTATAGCAAAAGCGCTTGGTGACATAGCGCGTGCTAAAGGAATGGCCC
AAGTCGCCAAGGAATCTGGACTCTCAAGAGAAAGCTTATACAAAGCCCTATCTGGTGATCGCATCCCGGGCTTTGATACG
GTATTAAAGGTACTTACAGCCCTTGGCCTTAGCCTGCACACAAAACCAATTAGTAAGATTCGTGCTTAA

Structures

Toxin

• AlphaFold DB: A0A5E1A1U8