Detailed information of TA system TA143653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 622538..623114
• Replicon: chromosome
• Accession: NZ_CP046116
• Organism: Enterobacter cloacae strain CBG15936

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: GJ694_RS02855
• Protein ID: WP_058683981.1
• Coordinates: 622538..622825 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A5E1AP18
• Locus tag: GJ694_RS02860
• Protein ID: WP_023620649.1
• Coordinates: 622812..623114 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ694_RS02830	617602..618297	+	696	WP_038418621.1	winged helix-turn-helix domain-containing protein	-
GJ694_RS02835	618500..618970	+	471	WP_013095432.1	MarR family transcriptional regulator	-
GJ694_RS02840	618967..620034	+	1068	WP_095440265.1	HlyD family secretion protein	-
GJ694_RS02845	620024..621100	+	1077	WP_013095434.1	DUF2955 domain-containing protein	-
GJ694_RS02850	621070..622368	-	1299	WP_129252076.1	DUF445 domain-containing protein	-
GJ694_RS02855	622538..622825	+	288	WP_058683981.1	BrnT family toxin	Toxin
GJ694_RS02860	622812..623114	+	303	WP_023620649.1	BrnA antitoxin family protein	Antitoxin
GJ694_RS02865	623145..623783	-	639	WP_129252074.1	LysE family translocator	-
GJ694_RS02870	623832..624575	-	744	WP_048972195.1	AraC family transcriptional regulator	-
GJ694_RS02875	624713..626083	+	1371	WP_129252072.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
GJ694_RS02880	626125..626445	-	321	WP_013095439.1	hypothetical protein	-
GJ694_RS02885	626445..626990	-	546	WP_013095440.1	porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11291.64 Da
• Isoelectric Point: 6.4872

>T143653 WP_058683981.1 NZ_CP046116:622538-622825 [Enterobacter cloacae]
MPTEYEWDSDKAKSNQQKHDIRFEDAVLVFDDPYHLSVQERYENGEFRWQTIGLVQGVIVILVAHTVRFESGGEIIRIIS
ARKADRKERSRYEHR

Nucleotide

• Length: 288 bp

>T143653 NZ_CP061306:105919-106305 [Polynucleobacter sp. JS-JIR-II-b4]
ATGCTCGATACCAATACCATCAGCAGCCTGATTAAGAAAAACCCAGTAGTAAGCAGAAAAATCGCCAGTGTTCCTATGGA
AAGACTATGCTTGTCAGTTATATCTGAAGGTGAAATTCTCTATGGTTTGGCAAAAAAGCCAAATGCAAGGAACTTACATT
TGGTTGTACAAGAGTTTTTAAAGCGAGTGGATGTTTTGGTTTGGGATACTGATGTAGCGGAGCATTATGGTGTTTTAAGG
GCTCAATTAGAGAGTAATGGAAATTTACTAGGCCCTCTTGACATGCAAATTGCAGCACATGCTTCTCAGCTCGGGGCAAT
TTTAGTAACAAATGACCAAGCTTTTAAAAGGATTCAGAAGCTACAGGTAGAGGATTGGACTGTATAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11414.05 Da
• Isoelectric Point: 10.3694

>AT143653 WP_023620649.1 NZ_CP046116:622812-623114 [Enterobacter cloacae]
MSIVKHKRGSSPEVSARRDAELKALAEKPEAEIDYSDIPATDDKQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT143653 NZ_CP061306:105650-105910 [Polynucleobacter sp. JS-JIR-II-b4]
ATGAGCCTAATAGCAAAAATCTTTATGAATGGACGCAGCCAGGCCATACGCTTGCCTGCGGCATTTCGTTTCGAGGAAAA
AGAGGTTTTTATTCGGCGCGATGCTAATACAGGTGATTTAATTCTTTCCAGAAGACCTGAAAACTGGGATAGTTTTTTCT
TGGCGCTTGCGAGCGCGGATGTTCCTTTGGATTTCTTAAGCCCTAAAGATCGAAATCAAGGTTCGCAAGATCGAGATCCT
TTTGCTGGAGTCAATGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A5E1AP18