Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 178622..179226 Replicon chromosome
Accession NZ_CP046116
Organism Enterobacter cloacae strain CBG15936

Toxin (Protein)


Gene name hicA Uniprot ID A0A421IJD5
Locus tag GJ694_RS00860 Protein ID WP_071843252.1
Coordinates 178622..178807 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A5E1AFC9
Locus tag GJ694_RS00865 Protein ID WP_023621194.1
Coordinates 178822..179226 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJ694_RS00845 175053..176591 + 1539 WP_020686520.1 aldehyde dehydrogenase -
GJ694_RS00850 176588..177553 - 966 WP_061771396.1 LysR family transcriptional regulator -
GJ694_RS00855 177671..178411 + 741 WP_013094942.1 MipA/OmpV family protein -
GJ694_RS00860 178622..178807 + 186 WP_071843252.1 type II toxin-antitoxin system HicA family toxin Toxin
GJ694_RS00865 178822..179226 + 405 WP_023621194.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GJ694_RS00870 179280..181235 - 1956 WP_100168001.1 glycoside hydrolase family 127 protein -
GJ694_RS00875 181246..182646 - 1401 WP_020686524.1 MFS transporter -
GJ694_RS00880 182871..183689 + 819 WP_020686525.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6859.08 Da        Isoelectric Point: 11.7891

>T143652 WP_071843252.1 NZ_CP046116:178622-178807 [Enterobacter cloacae]
VKSADVITVLVRHGWKCVRTKGSHHQFRHPVHAGLVTVPHPKKDIKPGTLAQIWRQAGIKH

Download         Length: 186 bp

>T143652 NZ_CP061306:c105039-104914 [Polynucleobacter sp. JS-JIR-II-b4]
ATGAATAGCAAAGAACTCATCAAGACATTAGAAAAAGATGGCTGGGTATTGCGGGGCTCAAAAGGCTCTCATCATGTTTT
TAACCATCCCCGCAAGGCTGGACACATTACCGTTCCATACCCTTAA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14888.72 Da        Isoelectric Point: 4.3036

>AT143652 WP_023621194.1 NZ_CP046116:178822-179226 [Enterobacter cloacae]
MFYPAYIHSDPDGSASGFFPDVPGCFFAGNSLDDAFQDARDALTAHFEALFEMDEELPLPGNVEAHLEATPGDFIGGQWL
LVDINMKQFDGKAERINITLPRRLLVKIDSFVSEHPQFSNRSAFLAEAARRVLP

Download         Length: 405 bp

>AT143652 NZ_CP061306:c104845-104453 [Polynucleobacter sp. JS-JIR-II-b4]
ATGAAATATCCAATTGCTATTGAACCAGGTGGAGATAAGTTGGCATGGAGCGTCATCGTTCCTGATATGCCAGGCTGTTT
CTCGGCTGCTGACAGCGGTATTGACGAGGCTATTGAAAATGCCAAAGAAGCGATTGGGCTTTGGATTGAAACCGCCCTAG
ATGCGGGCGAAGATATACCCAAGCCCAGCTCCATTACCGATCTACAGAAGAAGAAAGTGTTCAAGGGATTTATCTGGGCA
ATTGCCGAGATAAATCCATCTTTGCTATCAGACGATATTGAAAGAGTAAATATCTCTTTACCAAAAAGAGTATTGGCAAG
ATTAGATGCCAAAGCTAAATCCGCTGGTGAAAATCGCTCTGCTTTTATCGCGCATATGGCGATTAGCGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A421IJD5

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AFC9

References