Detailed information of TA system TA143649

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 4121400..4122133
• Replicon: chromosome
• Accession: NZ_CP046115
• Organism: Klebsiella oxytoca strain P620

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: GJ746_RS19160
• Protein ID: WP_154681611.1
• Coordinates: 4121804..4122133 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: GJ746_RS19155
• Protein ID: WP_154681610.1
• Coordinates: 4121400..4121765 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ746_RS19110	4116696..4117400	+	705	WP_154681606.1	WYL domain-containing protein	-
GJ746_RS19115	4117400..4117969	+	570	WP_154682775.1	hypothetical protein	-
GJ746_RS19120	4118006..4118458	+	453	WP_099433365.1	hypothetical protein	-
GJ746_RS19125	4118455..4118904	+	450	WP_154681607.1	hypothetical protein	-
GJ746_RS19130	4118976..4119206	+	231	WP_154681608.1	DUF905 domain-containing protein	-
GJ746_RS19135	4119305..4120126	+	822	WP_154682776.1	DUF945 domain-containing protein	-
GJ746_RS19140	4120158..4120601	+	444	WP_063963756.1	antirestriction protein	-
GJ746_RS19145	4120614..4121156	+	543	WP_154681609.1	DNA repair protein RadC	-
GJ746_RS19150	4121153..4121374	+	222	WP_012543032.1	DUF987 domain-containing protein	-
GJ746_RS19155	4121400..4121765	+	366	WP_154681610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GJ746_RS19160	4121804..4122133	+	330	WP_154681611.1	TA system toxin CbtA family protein	Toxin
GJ746_RS19165	4122736..4123467	+	732	WP_154681612.1	GntR family transcriptional regulator	-
GJ746_RS19170	4123464..4123901	+	438	WP_154681613.1	PTS fructose transporter subunit IIA	-
GJ746_RS19175	4123904..4124686	+	783	WP_154681614.1	PTS sugar transporter subunit IIC	-
GJ746_RS19180	4124679..4125458	+	780	WP_154682777.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
GJ746_RS19185	4125487..4125975	+	489	WP_154681615.1	PTS sugar transporter subunit IIB	-
GJ746_RS19190	4125979..4126683	+	705	WP_154681616.1	sugar isomerase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4097785..4133419	35634

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12324.08 Da
• Isoelectric Point: 7.3215

>T143649 WP_154681611.1 NZ_CP046115:4121804-4122133 [Klebsiella oxytoca]
MQTLSSHPTRAAQPCLSPIEIWQCLLTHLLSQHYGLTLNDTPFSNETTIQEHIDAGISLSDAVNFLVEKYGLVRIDRKGF
SWQEQTPYLSVVDILRARRSTGLLKTNVK

Nucleotide

• Length: 330 bp

>T143649 NZ_CP061302:547237-547500 [Polynucleobacter sp. MG-Unter2-18]
ATGCTGAGTAGGGTTGTATGGACAAGCGCTGCATGGTCTGATTACGTTTACTGGCAAGGACAAGATAAAAAAACTTTAAG
GCGTATCAACGCACTTATAAAAGATGTCATGCGAAATCCTGAGGATGGTATTGGAAAGCCTGAGGAATTAAGGGAAAGTC
TGTCGGGATTTTGGTCTAGAAGAATTGATGATGTTCATCGCCTTGTCTACGCTATTGAGAAAGATCAACTTGTGATTATT
GCCTGCCGATATCACTATCAATAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13470.16 Da
• Isoelectric Point: 5.8720

>AT143649 WP_154681610.1 NZ_CP046115:4121400-4121765 [Klebsiella oxytoca]
MNNHSESGTKPDNPACQQWGLKRTITPCFGARLVQEGNRVHFLADRAGFNGAFSDGDVLHLDQAFPLILKQLELMLTSGE
LNPRYLHCVTFYHNGLTCEADTLGSCGYVYIAIYPDQTEPH

Nucleotide

• Length: 366 bp

>AT143649 NZ_CP061302:546990-547247 [Polynucleobacter sp. MG-Unter2-18]
ATGAGAGTGATCAATTTTTCTGACGCAAGAAACCAATTTAAGCAAGTAATCGATCAAGTGGTAAATGATTGTGATGTAGC
CATCATCTCTCGCAGGGATGCTGATGATGCTGTAGTGATGTCTTTGAATACCTATAACAGCATGATGGAAACACTCCATT
TATTAAAATCTCCAGCTAACGTAAAACATTTGGAGAAATCATTAGCGCAATATCGAAAAGGGAAAACCAAAGAAAAGGCT
CTTGTAGATGCTGAGTAG