Detailed information of TA system TA143640

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1360169..1360788
• Replicon: chromosome
• Accession: NZ_CP046115
• Organism: Klebsiella oxytoca strain P620

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: H3N9D8
• Locus tag: GJ746_RS06345
• Protein ID: WP_004099646.1
• Coordinates: 1360169..1360387 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: GJ746_RS06350
• Protein ID: WP_154679420.1
• Coordinates: 1360414..1360788 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ746_RS06315	1356224..1356487	+	264	WP_154679416.1	type B 50S ribosomal protein L31	-
GJ746_RS06320	1356487..1356627	+	141	WP_003859006.1	type B 50S ribosomal protein L36	-
GJ746_RS06325	1356624..1357322	-	699	WP_154679417.1	GNAT family N-acetyltransferase	-
GJ746_RS06330	1357423..1358877	+	1455	WP_154682658.1	PLP-dependent aminotransferase family protein	-
GJ746_RS06335	1358852..1359322	-	471	WP_154679418.1	YlaC family protein	-
GJ746_RS06340	1359440..1360006	-	567	WP_154679419.1	maltose O-acetyltransferase	-
GJ746_RS06345	1360169..1360387	-	219	WP_004099646.1	hemolysin expression modulator Hha	Toxin
GJ746_RS06350	1360414..1360788	-	375	WP_154679420.1	Hha toxicity modulator TomB	Antitoxin
GJ746_RS06355	1361272..1364418	-	3147	WP_154679421.1	multidrug efflux RND transporter permease subunit AcrB	-
GJ746_RS06360	1364441..1365634	-	1194	WP_154679422.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8640.05 Da
• Isoelectric Point: 8.9008

>T143640 WP_004099646.1 NZ_CP046115:c1360387-1360169 [Klebsiella oxytoca]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Nucleotide

• Length: 219 bp

>T143640 NZ_CP061298:325826-326095 [Polynucleobacter paludilacus]
TTGGCCTGGATCATTAAGTACACAGAGTCTTCAAGTAAGCAGCTCAAAAAATTAGATAAGCAAACTGCATTACGCGTACT
AGATTACATGGATGAGCGCGTTGCGGTATTGAATGACCCACGTTCTTTGGGTAAAAATTTAAAAGGCCCCAAGATAGGTG
AATATTGGCGGTACAGAGTGGGCGATATTAGAGTGATCTGCAATATCGTAGACGGACAGATGATGGTCTTAGTGATCGAG
ATTGGAAATCGTCGTGAAGTTTATAGATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14364.11 Da
• Isoelectric Point: 4.8989

>AT143640 WP_154679420.1 NZ_CP046115:c1360788-1360414 [Klebsiella oxytoca]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQLDEYL
DDTFVLFSNYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT143640 NZ_CP061298:325615-325842 [Polynucleobacter paludilacus]
ATGGCTGTTTCAATTCGGCTTGATCCAGAGATTGAGCAAAGATTAGATCAACTAGCTGCACAAACTGGTAGGGCTAAATC
GTATTATTTGCGTGAGTTGATCGAGGGTGGGTTGGATGACCTTGAAGATTTCTATTTGGCAGATGCGGTAATGGAGCGGG
TGCGCAGAGGCAGCGAAAAACTATTAGATGCTACCCAGGTAAGGAAAGATCTTGGCCTGGATCATTAA

Structures

Toxin

• AlphaFold DB: A0A0H3H713