Detailed information of TA system TA143633

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 1158164..1158805
• Replicon: chromosome
• Accession: NZ_CP046114
• Organism: Glaesserella parasuis strain SCW0912

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B8F4G6
• Locus tag: GJV39_RS06025
• Protein ID: WP_012621798.1
• Coordinates: 1158623..1158805 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GJV39_RS06020
• Protein ID: WP_021109631.1
• Coordinates: 1158164..1158583 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJV39_RS05985	1153278..1153922	-	645	WP_035498099.1	antirepressor	-
GJV39_RS05990	1154170..1155195	-	1026	WP_021109600.1	hypothetical protein	-
GJV39_RS05995	1155527..1156060	-	534	WP_010786726.1	hypothetical protein	-
GJV39_RS06000	1156316..1156558	-	243	WP_005713710.1	hypothetical protein	-
GJV39_RS06005	1156902..1157123	-	222	WP_020997039.1	hypothetical protein	-
GJV39_RS06010	1157138..1157323	+	186	WP_020997221.1	YegP family protein	-
GJV39_RS06015	1157349..1157636	-	288	WP_012621801.1	hypothetical protein	-
GJV39_RS06020	1158164..1158583	-	420	WP_021109631.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GJV39_RS06025	1158623..1158805	-	183	WP_012621798.1	type II toxin-antitoxin system HicA family toxin	Toxin
GJV39_RS06030	1158926..1159717	-	792	WP_021109630.1	DUF1828 domain-containing protein	-
GJV39_RS06035	1159717..1160208	-	492	WP_021109629.1	hypothetical protein	-
GJV39_RS06040	1160260..1160925	-	666	WP_021109628.1	helix-turn-helix transcriptional regulator	-
GJV39_RS06045	1161070..1161285	+	216	WP_035500508.1	helix-turn-helix domain-containing protein	-
GJV39_RS06050	1161565..1162347	+	783	WP_021109626.1	Rha family transcriptional regulator	-
GJV39_RS06055	1162344..1163174	+	831	WP_035497995.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1128230..1195781	67551

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6883.01 Da
• Isoelectric Point: 10.2994

>T143633 WP_012621798.1 NZ_CP046114:c1158805-1158623 [Glaesserella parasuis]
MKYSEFLRYLLAQGCEIETQKRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Nucleotide

• Length: 183 bp

>T143633 NZ_CP061296:283745-284143 [Polynucleobacter sp. MWH-P3-07-1]
ATGATCAAGTATTTACTTGATACCAATATCGTGATTTATGTTCTCAAGCGCAGGCCCATTGAGGCCCTGGAAACCTTTAA
TAAAAATGCCAATCGTATCGCAATTTCAAGCATCACCCTCTCAGAGCTCATCTATGGCGCTGAAAAGAGCGCCAATGTCG
ATAAAAATCTTGAGGCTGTTGAGGATTTTGTGAGCCACCTTGATGTTTTTTCCTATGACGCTAAAGCATCCCAGCAGTAT
GGGCAAATTAAAGCTGGCTTAGAGAAGCGTGGGGAACTTATCGGAGAGAATGACATTCATATTGCTGCCCATGCAATTAG
CCAGGGCCTCATTCTAGTCAGTAACAATTTAAGAGAGTTCAAAAGGGTGCCAAACCTTGCGTTAGAGAATTGGGTTTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 16051.53 Da
• Isoelectric Point: 5.2662

>AT143633 WP_021109631.1 NZ_CP046114:c1158583-1158164 [Glaesserella parasuis]
MLRYPVEITKDDNNTYLVTCPDIPEMASVGEDIEEALLEAKDGLAAALEFYFDDRRTIPLPSPVKEGQHTVDLSILQSMK
VFLLNEMIKQDVRKAEMARRLDVHLPQIDRLLDFKHATKVEFVEKAYSKLNRHFTILPH

Nucleotide

• Length: 420 bp

>AT143633 NZ_CP061296:283512-283745 [Polynucleobacter sp. MWH-P3-07-1]
ATTACTACTTCCACAGTTTTTACCAACAATCGCTCTCAAGCAGTGCGTCTACCCACTGAGGCTCGTTTACCTGATGCGGT
GAAAAAGGTCATTGTGCGCACCCGTGGCCACGAGAGGATCATCACCCCTATTGAAAATACCTGGGACCATTTCTTTCTTA
GCGGCCCACTAGTGACTGACGACTTTATGAGCGAGCGTGGAGAGCAAAAACCTGCAGTTCGAGAGTCTCTCTGA

Structures

Toxin

• AlphaFold DB: B8F4G6