Detailed information of TA system TA143587

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2547248..2547819
• Replicon: chromosome
• Accession: NZ_CP046112
• Organism: Enterococcus faecalis strain 111540027-2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GKQ55_RS12230
• Protein ID: WP_002354774.1
• Coordinates: 2547248..2547589 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: GKQ55_RS12235
• Protein ID: WP_002354773.1
• Coordinates: 2547589..2547819 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKQ55_RS12225	2543263..2546877	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
GKQ55_RS12230	2547248..2547589	-	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GKQ55_RS12235	2547589..2547819	-	231	WP_002354773.1	hypothetical protein	Antitoxin
GKQ55_RS12240	2547994..2548209	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
GKQ55_RS12245	2548348..2549340	+	993	WP_142426550.1	biotin--[acetyl-CoA-carboxylase] ligase	-
GKQ55_RS12250	2549604..2550233	-	630	WP_002354763.1	lytic polysaccharide monooxygenase	-
GKQ55_RS12255	2550926..2552542	+	1617	WP_002381036.1	phosphatase PAP2/LCP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T143587 WP_002354774.1 NZ_CP046112:c2547589-2547248 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T143587 NZ_CP061207:75899-76186 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAAATGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGCGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT143587 WP_002354773.1 NZ_CP046112:c2547819-2547589 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT143587 NZ_CP061207:75651-75902 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTGGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812