Detailed information of TA system TA143576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 1584490..1585184
• Replicon: chromosome
• Accession: NZ_CP046112
• Organism: Enterococcus faecalis strain 111540027-2

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: S4BLV4
• Locus tag: GKQ55_RS07815
• Protein ID: WP_002378467.1
• Coordinates: 1584840..1585184 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: GKQ55_RS07810
• Protein ID: WP_002392358.1
• Coordinates: 1584490..1584822 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKQ55_RS07765	1579898..1580215	-	318	WP_002357007.1	hypothetical protein	-
GKQ55_RS07770	1580435..1580989	+	555	WP_002357006.1	hypothetical protein	-
GKQ55_RS07775	1581274..1581612	-	339	WP_033783419.1	hypothetical protein	-
GKQ55_RS07780	1581649..1581858	-	210	WP_002399426.1	hypothetical protein	-
GKQ55_RS07785	1581913..1582101	+	189	WP_002364350.1	YegP family protein	-
GKQ55_RS07790	1582127..1582852	-	726	WP_002364352.1	phage regulatory protein	-
GKQ55_RS07795	1582891..1583202	-	312	WP_002381719.1	hypothetical protein	-
GKQ55_RS07800	1583799..1583996	-	198	WP_010712611.1	hypothetical protein	-
GKQ55_RS07805	1584009..1584191	-	183	WP_002358110.1	hypothetical protein	-
GKQ55_RS07810	1584490..1584822	+	333	WP_002392358.1	helix-turn-helix domain-containing protein	Antitoxin
GKQ55_RS07815	1584840..1585184	+	345	WP_002378467.1	ImmA/IrrE family metallo-endopeptidase	Toxin
GKQ55_RS07820	1585219..1585947	+	729	WP_010784612.1	potassium channel family protein	-
GKQ55_RS07825	1586047..1587195	+	1149	WP_002388210.1	site-specific integrase	-
GKQ55_RS07830	1587223..1587666	-	444	WP_002388212.1	hypothetical protein	-
GKQ55_RS07835	1587663..1587938	-	276	WP_002364235.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
GKQ55_RS07840	1587938..1588984	-	1047	WP_002403888.1	type II secretion system F family protein	-
GKQ55_RS07845	1588941..1589909	-	969	WP_002364237.1	Flp pilus assembly complex ATPase component TadA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (22-89)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-59)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13712.62 Da
• Isoelectric Point: 5.5523

>T143576 WP_002378467.1 NZ_CP046112:1584840-1585184 [Enterococcus faecalis]
MKSIKELVEEYEVELVFAPINKRACYEPVKRIIFVNQNLSIEEQEESIFHEFKHVVSHSDYIELYKIPSFRNKMEAEADY
HMFKCLIEKHDGQFNYSNVITHYNLKMGQETYLN

Nucleotide

• Length: 345 bp

>T143576 NZ_CP061206:3292135-3292446 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCTTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATCACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12660.01 Da
• Isoelectric Point: 4.5116

>AT143576 WP_002392358.1 NZ_CP046112:1584490-1584822 [Enterococcus faecalis]
MNTYEIIKDLAKEKGISIRQLEINFGYSNGYIGSWKRQTPNSNELSRLADYFDVSVDYLLGRTDNTKATDEKKSDDLDDV
LDNVMSFDGEPLDDHDREVIRAYLKGRFGK

Nucleotide

• Length: 333 bp

>AT143576 NZ_CP061206:3292443-3292862 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCAGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATACCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: S4BLV4