Detailed information of TA system TA143571

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 1352009..1352703
• Replicon: chromosome
• Accession: NZ_CP046111
• Organism: Enterococcus faecalis strain 111540047-1

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: S4BLV4
• Locus tag: GKQ56_RS06415
• Protein ID: WP_002378467.1
• Coordinates: 1352009..1352353 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: GKQ56_RS06420
• Protein ID: WP_002392358.1
• Coordinates: 1352371..1352703 (-)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKQ56_RS06385	1347284..1348252	+	969	WP_002364237.1	Flp pilus assembly complex ATPase component TadA	-
GKQ56_RS06390	1348209..1349255	+	1047	WP_002403888.1	type II secretion system F family protein	-
GKQ56_RS06395	1349255..1349530	+	276	WP_002364235.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
GKQ56_RS06400	1349527..1349970	+	444	WP_002388212.1	hypothetical protein	-
GKQ56_RS06405	1349998..1351146	-	1149	WP_002388210.1	site-specific integrase	-
GKQ56_RS06410	1351246..1351974	-	729	WP_010784612.1	potassium channel family protein	-
GKQ56_RS06415	1352009..1352353	-	345	WP_002378467.1	ImmA/IrrE family metallo-endopeptidase	Toxin
GKQ56_RS06420	1352371..1352703	-	333	WP_002392358.1	helix-turn-helix domain-containing protein	Antitoxin
GKQ56_RS06425	1353002..1353184	+	183	WP_002358110.1	hypothetical protein	-
GKQ56_RS06430	1353197..1353394	+	198	WP_010712611.1	hypothetical protein	-
GKQ56_RS06435	1353991..1354302	+	312	WP_002381719.1	hypothetical protein	-
GKQ56_RS06440	1354341..1355066	+	726	WP_002364352.1	phage regulatory protein	-
GKQ56_RS06445	1355092..1355280	-	189	WP_002364350.1	YegP family protein	-
GKQ56_RS06450	1355335..1355544	+	210	WP_002399426.1	hypothetical protein	-
GKQ56_RS06455	1355581..1355919	+	339	WP_033783419.1	hypothetical protein	-
GKQ56_RS06460	1356204..1356758	-	555	WP_002357006.1	hypothetical protein	-
GKQ56_RS06465	1356978..1357295	+	318	WP_002357007.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (22-89)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-59)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13712.62 Da
• Isoelectric Point: 5.5523

>T143571 WP_002378467.1 NZ_CP046111:c1352353-1352009 [Enterococcus faecalis]
MKSIKELVEEYEVELVFAPINKRACYEPVKRIIFVNQNLSIEEQEESIFHEFKHVVSHSDYIELYKIPSFRNKMEAEADY
HMFKCLIEKHDGQFNYSNVITHYNLKMGQETYLN

Nucleotide

• Length: 345 bp

>T143571 NZ_CP061206:c2164507-2164082 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCTCCTGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCAATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGGTCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTGCGGGCGCGGCAGGCGACCGGCCTGCGGATTCAGTG
CAAAAACACGAATATAATCAGCCGGATGGCTGATTCAAGACATGATAAAAGACTTTACCAGCGCACGTTTAAAGCGTGCA
AGAGATGTTATTATACTAAAACATAA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12660.01 Da
• Isoelectric Point: 4.5116

>AT143571 WP_002392358.1 NZ_CP046111:c1352703-1352371 [Enterococcus faecalis]
MNTYEIIKDLAKEKGISIRQLEINFGYSNGYIGSWKRQTPNSNELSRLADYFDVSVDYLLGRTDNTKATDEKKSDDLDDV
LDNVMSFDGEPLDDHDREVIRAYLKGRFGK

Nucleotide

• Length: 333 bp

>AT143571 NZ_CP061206:c2164873-2164538 [Escherichia coli]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGTTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCCCCTGCCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Toxin

• AlphaFold DB: S4BLV4