Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-dinJ/YafQ-DinJ
Location 11429..11963 Replicon plasmid pTEF2
Accession NZ_CP046110
Organism Enterococcus faecalis strain 133170041-3

Toxin (Protein)


Gene name relE Uniprot ID R3JX52
Locus tag GKQ57_RS14410 Protein ID WP_002360769.1
Coordinates 11688..11963 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID R3H6P0
Locus tag GKQ57_RS14405 Protein ID WP_002369771.1
Coordinates 11429..11695 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GKQ57_RS14370 7287..8072 + 786 WP_002360778.1 replication-relaxation family protein -
GKQ57_RS14375 8078..8566 + 489 WP_002412099.1 hypothetical protein -
GKQ57_RS14380 9158..9424 + 267 WP_002360776.1 hypothetical protein -
GKQ57_RS14385 9457..9957 + 501 WP_002360775.1 DnaJ domain-containing protein -
GKQ57_RS14390 9970..10218 + 249 WP_002360773.1 DUF3850 domain-containing protein -
GKQ57_RS14395 10294..10710 + 417 WP_002414801.1 single-stranded DNA-binding protein -
GKQ57_RS14400 10734..11318 + 585 WP_002383617.1 thermonuclease family protein -
GKQ57_RS14405 11429..11695 + 267 WP_002369771.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
GKQ57_RS14410 11688..11963 + 276 WP_002360769.1 type II toxin-antitoxin system YafQ family toxin Toxin
GKQ57_RS14415 12768..12953 + 186 WP_002368099.1 hypothetical protein -
GKQ57_RS14420 12938..13129 + 192 WP_002383620.1 DpnD/PcfM family protein -
GKQ57_RS14425 13361..14140 + 780 WP_002383621.1 hypothetical protein -
GKQ57_RS14430 14140..15399 + 1260 WP_002360763.1 PcfJ domain-containing protein -
GKQ57_RS14435 15996..16724 + 729 WP_002383622.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..53448 53448


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(7-86)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10732.58 Da        Isoelectric Point: 9.6918

>T143540 WP_002360769.1 NZ_CP046110:11688-11963 [Enterococcus faecalis]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Download         Length: 276 bp

>T143540 NZ_CP061185:c4683474-4683289 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9772.09 Da        Isoelectric Point: 4.4791

>AT143540 WP_002369771.1 NZ_CP046110:11429-11695 [Enterococcus faecalis]
VATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Download         Length: 267 bp

>AT143540 NZ_CP061185:c4683272-4682862 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGCCGCGCACTTCGAAACCTTATGCGAGATGG
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2AEA7

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AED7

References