Detailed information of TA system TA143532

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/Peptidase_M78-HTH_26
• Location: 1258059..1258757
• Replicon: chromosome
• Accession: NZ_CP046108
• Organism: Enterococcus faecalis strain 133170041-3

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: A0A828QMF1
• Locus tag: GKQ57_RS05835
• Protein ID: WP_002388207.1
• Coordinates: 1258059..1258403 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: GKQ57_RS05840
• Protein ID: WP_002388206.1
• Coordinates: 1258422..1258757 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKQ57_RS05805	1253311..1254279	+	969	WP_002369168.1	Flp pilus assembly complex ATPase component TadA	-
GKQ57_RS05810	1254236..1255282	+	1047	WP_002369171.1	type II secretion system F family protein	-
GKQ57_RS05815	1255282..1255557	+	276	WP_002356991.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
GKQ57_RS05820	1255554..1255997	+	444	WP_016617790.1	hypothetical protein	-
GKQ57_RS05825	1256025..1257173	-	1149	WP_002388210.1	site-specific integrase	-
GKQ57_RS05830	1257273..1258001	-	729	WP_002388208.1	ion transporter	-
GKQ57_RS05835	1258059..1258403	-	345	WP_002388207.1	ImmA/IrrE family metallo-endopeptidase	Toxin
GKQ57_RS05840	1258422..1258757	-	336	WP_002388206.1	helix-turn-helix domain-containing protein	Antitoxin
GKQ57_RS05845	1259049..1259231	+	183	WP_002388205.1	hypothetical protein	-
GKQ57_RS05850	1259244..1259441	+	198	WP_010712611.1	hypothetical protein	-
GKQ57_RS05855	1260038..1260349	+	312	WP_002381719.1	hypothetical protein	-
GKQ57_RS05860	1260372..1261094	+	723	WP_002357000.1	phage regulatory protein	-
GKQ57_RS05865	1261120..1261308	-	189	WP_002357001.1	YegP family protein	-
GKQ57_RS05870	1261363..1261572	+	210	WP_002357002.1	hypothetical protein	-
GKQ57_RS05875	1261609..1261947	+	339	WP_002357003.1	hypothetical protein	-
GKQ57_RS05880	1262232..1262786	-	555	WP_002357006.1	hypothetical protein	-
GKQ57_RS05885	1263006..1263323	+	318	WP_002357007.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1255581..1306528	50947

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (21-86)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-60)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13625.58 Da
• Isoelectric Point: 4.9570

>T143532 WP_002388207.1 NZ_CP046108:c1258403-1258059 [Enterococcus faecalis]
MKSIKELVEEYNVELVFTTLNKRACFDPTYGIIFVNQNLTPTEQEEAIYHELKHVEDHVDIMALYKIPVFRSKMESEAEQ
YMFRSLIEKYEGQYNYSNVIAHYNLKMGQEIYLK

Nucleotide

• Length: 345 bp

>T143532 NZ_CP061185:c2860099-2859848 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TGCTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12842.51 Da
• Isoelectric Point: 5.2926

>AT143532 WP_002388206.1 NZ_CP046108:c1258757-1258422 [Enterococcus faecalis]
MTVFDRIKLLAKERGKSLNRIEEELGLPKNVLYRMKTSDNPTKDRLETLANYFDVSVDYLLGRTDNTKATDEKKSDDLDD
VLDNVMSFDGKPLDDHDREVIRAYLKGRFGK

Nucleotide

• Length: 336 bp

>AT143532 NZ_CP061185:c2859851-2859597 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A828QMF1