Detailed information of TA system TA143531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 588688..589724
• Replicon: chromosome
• Accession: NZ_CP046108
• Organism: Enterococcus faecalis strain 133170041-3

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: -
• Locus tag: GKQ57_RS02730
• Protein ID: WP_002371439.1
• Coordinates: 588688..589341 (-)
• Length: 218 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: R3K8V7
• Locus tag: GKQ57_RS02735
• Protein ID: WP_002371437.1
• Coordinates: 589338..589724 (-)
• Length: 129 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKQ57_RS02700	584328..584657	-	330	WP_002365236.1	helix-turn-helix transcriptional regulator	-
GKQ57_RS02705	584879..585460	+	582	WP_002380883.1	phosphoglycerate mutase family protein	-
GKQ57_RS02710	585477..585896	+	420	WP_002371441.1	MerR family transcriptional regulator	-
GKQ57_RS02715	585911..586846	+	936	WP_171803825.1	aldo/keto reductase	-
GKQ57_RS02720	586945..588276	+	1332	WP_002371440.1	FAD-dependent oxidoreductase	-
GKQ57_RS02725	588314..588649	-	336	WP_002365231.1	helix-turn-helix transcriptional regulator	-
GKQ57_RS02730	588688..589341	-	654	WP_002371439.1	ImmA/IrrE family metallo-endopeptidase	Toxin
GKQ57_RS02735	589338..589724	-	387	WP_002371437.1	helix-turn-helix domain-containing protein	Antitoxin
GKQ57_RS02740	590477..590728	+	252	WP_002365227.1	hypothetical protein	-
GKQ57_RS02745	590814..591140	+	327	WP_002371436.1	hypothetical protein	-
GKQ57_RS02750	591140..591538	+	399	WP_002371435.1	DUF961 family protein	-
GKQ57_RS02755	591726..591914	-	189	WP_002371434.1	hypothetical protein	-
GKQ57_RS02760	592141..592293	+	153	WP_002371433.1	hypothetical protein	-
GKQ57_RS02765	592334..592627	+	294	WP_029645162.1	hypothetical protein	-
GKQ57_RS02770	592687..593013	+	327	WP_002393071.1	nucleotidyltransferase domain-containing protein	-
GKQ57_RS02775	593003..593383	+	381	WP_002371429.1	DUF86 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	520708..595083	74375

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (78-181)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-64)

Sequences

Toxin

Protein

• Length: 218 a.a.
• Molecular weight: 26050.98 Da
• Isoelectric Point: 6.2394

>T143531 WP_002371439.1 NZ_CP046108:c589341-588688 [Enterococcus faecalis]
MIGLLLVDFISKELYFEAFNFSNELIKEVSYYSSKNIEQVKCFDIEKYVKEVEDVEFVEYTFQKQLKRRMLGSISKVDDE
VIVTTNKELMLERKNFTKMHEVMHYYIDIPKINNATHTFSDILLKNGYLMEDFPKEYRANVGASMLMANDQALFYALKKF
YSFEEIAQYFFMSKSALRNRLIEHLMYVNNCTFAHANTLFNNYYFHKETDIYRFIFN

Nucleotide

• Length: 654 bp

>T143531 NZ_CP061185:c2859851-2859597 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 129 a.a.
• Molecular weight: 15180.07 Da
• Isoelectric Point: 4.9147

>AT143531 WP_002371437.1 NZ_CP046108:c589724-589338 [Enterococcus faecalis]
VTIIKTNERLKQLRENKELTQKELADLLHMDRSVYNKIESGARPIRDNELIQFADFYNVSTDYLTNRTNNPIPPEEKTNV
GQNIISHFRLNTSDMDIEDIEELEEELIDFQDFLIKKAKEKKERNKKN

Nucleotide

• Length: 387 bp

>AT143531 NZ_CP061185:c2860099-2859848 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TGCTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: R3K8V7