Detailed information of TA system TA143522

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 43337..43872
• Replicon: plasmid unnamed1
• Accession: NZ_CP046100
• Organism: Rhodococcus sp. AQ5-07

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GJB56_RS29510
• Protein ID: WP_113683897.1
• Coordinates: 43337..43636 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GJB56_RS29515
• Protein ID: WP_113683896.1
• Coordinates: 43636..43872 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJB56_RS29495	39481..40551	+	1071	WP_113683900.1	DUF1016 family protein	-
GJB56_RS29500	40661..41458	-	798	WP_113683899.1	hypothetical protein	-
GJB56_RS29505	41458..43308	-	1851	WP_113683898.1	hypothetical protein	-
GJB56_RS29510	43337..43636	-	300	WP_113683897.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GJB56_RS29515	43636..43872	-	237	WP_113683896.1	antitoxin	Antitoxin
GJB56_RS29520	44306..44971	-	666	WP_113683895.1	TetR/AcrR family transcriptional regulator	-
GJB56_RS29525	45043..46017	-	975	WP_113683894.1	DUF2637 domain-containing protein	-
GJB56_RS29530	46658..46972	-	315	WP_113683893.1	hypothetical protein	-
GJB56_RS29535	46969..47751	-	783	WP_007733691.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..214483	214483

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 10485.01 Da
• Isoelectric Point: 4.5144

>T143522 WP_113683897.1 NZ_CP046100:c43636-43337 [Rhodococcus sp. AQ5-07]
MRPIHEANLDTVRPVLVLTREVVRPHLGTVTVAPITDEIRGLSTEVSVSTANGISADAVVSCDHITTIPVSALGNIIGYL
LPAQEESLSIAVRAAFDLE

Nucleotide

• Length: 300 bp

>T143522 NZ_CP061185:c1127909-1127691 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8768.84 Da
• Isoelectric Point: 6.4825

>AT143522 WP_113683896.1 NZ_CP046100:c43872-43636 [Rhodococcus sp. AQ5-07]
MSTQIAVRLPDEIVEFLDDEVREHRATSRASLVLRALERERRRQIAARDAEILSAATSEHDLSALAHHAVNRQPTGLT

Nucleotide

• Length: 237 bp

>AT143522 NZ_CP061185:c1128309-1127935 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG