Detailed information of TA system TA143521

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 35698..36647
• Replicon: plasmid unnamed1
• Accession: NZ_CP046100
• Organism: Rhodococcus sp. AQ5-07

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: GJB56_RS29480
• Protein ID: WP_113683903.1
• Coordinates: 35698..36273 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: GJB56_RS29485
• Protein ID: WP_113683902.1
• Coordinates: 36270..36647 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJB56_RS29465	31480..31950	-	471	WP_162793442.1	GtrA family protein	-
GJB56_RS29470	31950..32663	-	714	WP_154606712.1	anti-sigma factor	-
GJB56_RS29475	33891..35083	-	1193	Protein_27	IS110 family transposase	-
GJB56_RS29480	35698..36273	-	576	WP_113683903.1	zeta toxin family protein	Toxin
GJB56_RS29485	36270..36647	-	378	WP_113683902.1	hypothetical protein	Antitoxin
GJB56_RS29490	37232..39223	-	1992	WP_113683901.1	cutinase family protein	-
GJB56_RS29495	39481..40551	+	1071	WP_113683900.1	DUF1016 family protein	-
GJB56_RS29500	40661..41458	-	798	WP_113683899.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..214483	214483
	-	flank	IS/Tn	-	-	33891..34739	848

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-153)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (7-61)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21080.05 Da
• Isoelectric Point: 6.4997

>T143521 WP_113683903.1 NZ_CP046100:c36273-35698 [Rhodococcus sp. AQ5-07]
VRRLDLVVGPNGAGKSTFVEQRLTPLLPGSVFVNADVIAKDRWPDTAEEHSYEAARIAATMRGALIERGESFIAETVFSH
PSKLELIDHARRAGFVVVLHVVLVPVELSVARVAGRVVEGGHSVPEVKIRERFERLWSLVVDAIDRSDHATVYDNTAPDV
TRIVARFVDGDPVGEIAWPAWTPAEFRKAQK

Nucleotide

• Length: 576 bp

>T143521 NZ_CP061185:931859-932257 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13652.25 Da
• Isoelectric Point: 6.0954

>AT143521 WP_113683902.1 NZ_CP046100:c36647-36270 [Rhodococcus sp. AQ5-07]
MTEVVDKVTRFSSELVDAAAAEGQREHRSARQQLEHWARVGREVSNQRQVARQRVEAALAGRIPLNVLSAEEGAVFNAEI
AATLEESIATGNHVAERAVQGRATISLDEHGRVVKYLPDGSQIVL

Nucleotide

• Length: 378 bp

>AT143521 NZ_CP061185:931563-931856 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA