Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 527488..528059 Replicon chromosome
Accession NZ_CP046077
Organism Enterococcus faecium strain VRE

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag GJ652_RS03950 Protein ID WP_002286801.1
Coordinates 527718..528059 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag GJ652_RS03945 Protein ID WP_002323011.1
Coordinates 527488..527718 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJ652_RS03915 522621..523952 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
GJ652_RS03920 523974..524600 + 627 WP_002286816.1 cysteine hydrolase -
GJ652_RS03925 524783..525364 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
GJ652_RS03930 526096..526443 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
GJ652_RS03935 526419..526658 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
GJ652_RS03940 526863..527201 - 339 WP_002286804.1 hypothetical protein -
GJ652_RS03945 527488..527718 + 231 WP_002323011.1 hypothetical protein Antitoxin
GJ652_RS03950 527718..528059 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GJ652_RS03955 528908..529093 + 186 WP_002304207.1 hypothetical protein -
GJ652_RS03960 529598..529792 + 195 WP_002297028.1 hypothetical protein -
GJ652_RS03965 529950..530232 + 283 Protein_499 transposase -
GJ652_RS03970 530476..531306 - 831 WP_002286789.1 manganese catalase family protein -
GJ652_RS03975 531514..532848 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T143520 WP_002286801.1 NZ_CP046077:527718-528059 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T143520 NZ_CP061185:707976-708290 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAATC
CAATCCTTTTACTGGTAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCACACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT143520 WP_002323011.1 NZ_CP046077:527488-527718 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT143520 NZ_CP061185:707740-707973 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References