Detailed information of TA system TA143518

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 167437..168044
• Replicon: plasmid p5_03A17012
• Accession: NZ_CP046076
• Organism: Enterococcus faecium strain VRE

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: GJ652_RS01035
• Protein ID: WP_002321032.1
• Coordinates: 167437..167787 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: GJ652_RS01040
• Protein ID: WP_002287514.1
• Coordinates: 167781..168044 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ652_RS00995	162952..163221	-	270	WP_002389879.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GJ652_RS01000	163211..163477	-	267	WP_014748823.1	antitoxin	-
GJ652_RS01005	163763..164176	-	414	Protein_162	HAD-IA family hydrolase	-
GJ652_RS01010	164250..164930	+	681	WP_010861579.1	IS6-like element IS1216 family transposase	-
GJ652_RS01015	165055..165639	-	585	WP_002299575.1	recombinase family protein	-
GJ652_RS01020	165850..166053	-	204	WP_002299573.1	hypothetical protein	-
GJ652_RS01025	166063..166485	-	423	WP_002288787.1	hypothetical protein	-
GJ652_RS01030	166799..167413	+	615	WP_002318230.1	recombinase family protein	-
GJ652_RS01035	167437..167787	-	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GJ652_RS01040	167781..168044	-	264	WP_002287514.1	hypothetical protein	Antitoxin
GJ652_RS16675	168198..168362	+	165	WP_164450253.1	hypothetical protein	-
GJ652_RS01045	168400..169080	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
GJ652_RS01050	169137..169373	+	237	Protein_172	aminoglycoside phosphotransferase family protein	-
GJ652_RS01055	169357..169749	+	393	WP_025189010.1	GNAT family N-acetyltransferase	-
GJ652_RS01060	169750..171189	+	1440	WP_001028144.1	aminoglycoside O-phosphotransferase APH(2'')-Ia	-
GJ652_RS01065	171319..172491	-	1173	WP_000195429.1	IS256-like element IS256 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-aph(2'') / tet(L) / tet(M)	-	1..240928	240928
	-	inside	IScluster/Tn	aac(6')-aph(2'')	-	164250..172491	8241

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T143518 WP_002321032.1 NZ_CP046076:c167787-167437 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T143518 NZ_CP061185:344992-345165 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT143518 WP_002287514.1 NZ_CP046076:c168044-167781 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT143518 NZ_CP061185:344668-344964 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0