Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Txe-Axe/Txe-RelB
Location 7692..8211 Replicon plasmid p3_03A17012
Accession NZ_CP046075
Organism Enterococcus faecium strain VRE

Toxin (Protein)


Gene name Txe Uniprot ID Q848U6
Locus tag GJ652_RS00140 Protein ID WP_000588503.1
Coordinates 7954..8211 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name Axe Uniprot ID Q83WJ8
Locus tag GJ652_RS00135 Protein ID WP_000388479.1
Coordinates 7692..7961 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJ652_RS00100 3257..3673 - 417 WP_001232859.1 hypothetical protein -
GJ652_RS00105 3676..3960 - 285 WP_000353844.1 hypothetical protein -
GJ652_RS00110 4304..4549 + 246 WP_002311817.1 hypothetical protein -
GJ652_RS00115 4850..4936 - 87 WP_106914087.1 type I toxin-antitoxin system Fst family toxin -
GJ652_RS00120 5679..6359 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
GJ652_RS00125 6435..7001 + 567 Protein_8 hypothetical protein -
GJ652_RS00130 7382..7561 + 180 WP_001120991.1 hypothetical protein -
GJ652_RS00135 7692..7961 + 270 WP_000388479.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
GJ652_RS00140 7954..8211 + 258 WP_000588503.1 Txe/YoeB family addiction module toxin Toxin
GJ652_RS00145 9198..10517 - 1320 WP_000202380.1 IS1380-like element IS1678 family transposase -
GJ652_RS00150 10674..11120 + 447 Protein_13 hypothetical protein -
GJ652_RS00155 11275..12594 + 1320 WP_000202380.1 IS1380-like element IS1678 family transposase -
GJ652_RS00160 12798..13040 + 243 Protein_15 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..18356 18356
- inside IScluster/Tn - - 1657..12594 10937


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10206.53 Da        Isoelectric Point: 8.4369

>T143517 WP_000588503.1 NZ_CP046075:7954-8211 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Download         Length: 258 bp

>T143517 NZ_CP061181:c2286-2038 [Roseomonas sp. 546]
TTGATCCTGACCCGCCCCGCTCTGAAAGACCTCGGCAACGTGCCGAAGCGGGAGCGCGCTCAGCTGACCGCCCGCCTGGA
AGCCATCGCCGCCGCCCCGACCGGCAGCCACGGCAACGTCATTGCCATGAAGGGCGAGCCGGCCGGCCGCCTGCGGGTGC
GCCAGGGCGACTGGCGCGCCATCTTCACCATCGACGGCGACGATGTGGTAGTCATCCGCATCCGCCATCGCAGAGAGGTT
TACGACTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10373.51 Da        Isoelectric Point: 4.4346

>AT143517 WP_000388479.1 NZ_CP046075:7692-7961 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Download         Length: 270 bp

>AT143517 NZ_CP061181:c2038-1631 [Roseomonas sp. 546]
ATGGCCCACATTGGACCCCTGGCCGAGACGGCCGACACCGTGACGCTGCGGCGCGCGGACTTCGAAGCCCTGGTGGCGGC
GGCCGAGGACGTGGCCGACCGCGCCAGCTTCGCCGCCTTCGAGGCGCGCGTGGCGCGTGACGGCATGGCGGCGGTCAAGG
CGGATAGCCTGACCCTGCAGGAGGCGGTGCGCATCCTGGACGAAGGCGAAAGCCCGGTGCGGGTGTGGCGCGAGAAGCGC
GGGCTGACCGGCCGCGCCCTGGCCGCGGCAGCGGGTGTCAGCCCGGTGTATCTGTCGGAGATCGAGAACGGCAAGAAGCC
AGGCAGCGCCGCCGCCATCGTGGCGCTGGCCAAGGCGCTGCAGGTGGATGCCGGCGACCTGCTGCCGCCGGCCGCCACGG
ACGACTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZWU7

References