Detailed information of TA system TA143504

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 119301..120130
• Replicon: plasmid pTHAF191d_c
• Accession: NZ_CP046067
• Organism: Vibrio sp. THAF191d

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GGC04_RS25295
• Protein ID: WP_152472731.1
• Coordinates: 119301..119864 (-)
• Length: 188 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7Y3YYU7
• Locus tag: GGC04_RS25300
• Protein ID: WP_040122444.1
• Coordinates: 119861..120130 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GGC04_RS25280	114363..115331	-	969	WP_152472734.1	hypothetical protein	-
GGC04_RS25285	115463..115735	-	273	WP_152472733.1	hypothetical protein	-
GGC04_RS25290	115800..117893	-	2094	WP_152472732.1	TonB-dependent siderophore receptor	-
GGC04_RS25295	119301..119864	-	564	WP_152472731.1	GNAT family N-acetyltransferase	Toxin
GGC04_RS25300	119861..120130	-	270	WP_040122444.1	DUF1778 domain-containing protein	Antitoxin
GGC04_RS25305	120337..120759	-	423	WP_152472730.1	GNAT family N-acetyltransferase	-
GGC04_RS25310	120887..121291	-	405	WP_152472729.1	NUDIX domain-containing protein	-
GGC04_RS25315	121738..122214	-	477	WP_152472924.1	DUF1311 domain-containing protein	-
GGC04_RS25320	122381..122878	-	498	WP_152472728.1	GNAT family N-acetyltransferase	-
GGC04_RS25325	122940..123308	-	369	WP_152472727.1	hypothetical protein	-
GGC04_RS25330	123438..123815	-	378	WP_152472726.1	hypothetical protein	-
GGC04_RS25335	123951..124448	-	498	WP_152472725.1	DUF2867 domain-containing protein	-
GGC04_RS25340	124548..124880	-	333	WP_040122455.1	YnfA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..396761	396761

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-81)

Sequences

Toxin

Protein

• Length: 188 a.a.
• Molecular weight: 21099.61 Da
• Isoelectric Point: 9.3822

>T143504 WP_152472731.1 NZ_CP046067:c119864-119301 [Vibrio sp. THAF191d]
VSWGNEFVELSKSMHDRSSFDCGESELNTFIKTLAAKHMQAGISRTMVLPSSQPLLNQKYAICAFYSVAPSSISRETLPA
KLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKISLIRALKYLWEVNHHMRAYAIIVDCLTDSAQAFYTKFGFEVLCEHNG
RIRMFLPMKTVEQLFSLKATNITKDDV

Nucleotide

• Length: 564 bp

>T143504 NZ_CP061169:156474-156863 [Microbacterium sp. HY60]
GTGATCTACATCGACACCTCCGCGGTGGTGAAGACTCTGGTCTCCGAGAGAGGTAGTTCGTCTGTGCGGCGCCTCTTCGC
TACCTCAGAGCGGCTGGTCTCGTCCCGCCTTCTTGCCGTTGAGCTGAACACTGCAGCACAACGCCGAGGCCTCCCGGCCG
TCCACGTTGACGAGACGCTGAGTCGTGTGAACCTCGTCTCCGTTGATGACGACGTTCTCGACGGTGCCATTCGATTGCAG
TCTGGTTTGCGCGGCCTCGACGCCCTGCACCTTGCCACGGCAATTCTCATTGGCGACGAACTGACGGTATTTCTCGCCTT
TGACCCCGAGTTGAACGCGGCGGCGCGACGCTCCGGGCTCGCGCTCCACCCCGCATCAGCCGACGGCTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9839.34 Da
• Isoelectric Point: 4.5482

>AT143504 WP_040122444.1 NZ_CP046067:c120130-119861 [Vibrio sp. THAF191d]
MATARLDIRLDEEIKAKAEKASALLGLKSLTEYVVRLMDEDATQVIEQHESIVVKESVFDEFMMACDKAKAPNQALLDAA
KLTNESGIK

Nucleotide

• Length: 270 bp

>AT143504 NZ_CP061169:156214-156477 [Microbacterium sp. HY60]
ATGGGAAGCGTGACGCGCACAGAATTGAACCAGCAGACAGCACGGGTGCTGGCGCGCGTTGCGGCCGGTGAACGCGTGAC
GATCACCGACCGTGGTCGCCCCATCGCCGAGCTCACGCCGGCGCGCGAGTCGGGGTGGGATGCCCTGATTGCGGCGGGAA
AGGTTCGCACCGCGACGCGGTCGGGAAGCCTGACTTTCGAGCCGATCGACCTCGACGTTTCGTCGAGCGACATCATCGAC
GACATCAGGCGGGAGCGCGGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7Y3YYU7