Detailed information of TA system TA143501

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 6018253..6018839
• Replicon: chromosome
• Accession: NZ_CP046061
• Organism: Pseudomonas aeruginosa strain 1811-13R031

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: GJV01_RS28745
• Protein ID: WP_003120987.1
• Coordinates: 6018540..6018839 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: GJV01_RS28740
• Protein ID: WP_003448662.1
• Coordinates: 6018253..6018543 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJV01_RS28720	6013404..6013613	+	210	WP_003105733.1	cold-shock protein	-
GJV01_RS28725	6013835..6015724	+	1890	WP_016851610.1	hypothetical protein	-
GJV01_RS28730	6015721..6017697	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
GJV01_RS28735	6017838..6018182	+	345	WP_016851612.1	hypothetical protein	-
GJV01_RS28740	6018253..6018543	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
GJV01_RS28745	6018540..6018839	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GJV01_RS28750	6019041..6020165	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
GJV01_RS28755	6020165..6021874	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
GJV01_RS28760	6021878..6023203	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
GJV01_RS28765	6023193..6023726	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	5990185..6018839	28654

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T143501 WP_003120987.1 NZ_CP046061:c6018839-6018540 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T143501 NZ_CP061168:509401-509751 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT143501 WP_003448662.1 NZ_CP046061:c6018543-6018253 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT143501 NZ_CP061168:509114-509395 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: G8CP73