Detailed information of TA system TA143484

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT_toxin-BrnA
• Location: 4185765..4186257
• Replicon: chromosome
• Accession: NZ_CP046058
• Organism: Microcystis aeruginosa NIES-298

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: I4GAG4
• Locus tag: D3800_RS19725
• Protein ID: WP_002772034.1
• Coordinates: 4185765..4186043 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: D3800_RS19730
• Protein ID: WP_016514700.1
• Coordinates: 4186048..4186257 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3800_RS19705	4181739..4182347	-	609	WP_103111995.1	COP23 domain-containing protein	-
D3800_RS19710	4182407..4183507	-	1101	WP_103111996.1	S1C family serine protease	-
D3800_RS19715	4183651..4184145	-	495	WP_103111997.1	tetratricopeptide repeat protein	-
D3800_RS19720	4184451..4185407	-	957	WP_162205056.1	IS110 family transposase	-
D3800_RS19725	4185765..4186043	+	279	WP_002772034.1	BrnT family toxin	Toxin
D3800_RS19730	4186048..4186257	+	210	WP_016514700.1	BrnA antitoxin family protein	Antitoxin
D3800_RS19735	4186428..4188152	+	1725	WP_103111998.1	SUMF1/EgtB/PvdO family nonheme iron enzyme	-
D3800_RS19740	4188532..4188921	+	390	WP_002795690.1	nucleotidyltransferase family protein	-
D3800_RS19745	4188908..4189252	+	345	WP_103111999.1	DUF86 domain-containing protein	-
D3800_RS19750	4189382..4189630	+	249	WP_103112000.1	type II toxin-antitoxin system HicB family antitoxin	-
D3800_RS19755	4189920..4190105	-	186	WP_071590332.1	type II toxin-antitoxin system HicA family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (12-64)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10835.42 Da
• Isoelectric Point: 9.9743

>T143484 WP_002772034.1 NZ_CP046058:4185765-4186043 [Microcystis aeruginosa NIES-298]
MKYEWDQDKSDANLEKHGLSFEDAPLVFGGKCVTFEDSRFVYGEIRYITLGKLKGRVVLIVHTPRSNKTRIISMRKANNR
EQKIYQKRLEEN

Nucleotide

• Length: 279 bp

>T143484 NZ_CP061108:c4339022-4338849 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8174.07 Da
• Isoelectric Point: 3.9798

>AT143484 WP_016514700.1 NZ_CP046058:4186048-4186257 [Microcystis aeruginosa NIES-298]
MTDENIDYSDIPPLGDEFFTQETVSFSTSKQQLTIQLDQDILEWLKAQGKGYETQINYILREVMERQRQ

Nucleotide

• Length: 210 bp

>AT143484 NZ_CP061108:c4339346-4339050 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: I4GAG4