Detailed information of TA system TA143480

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1768848..1769629
• Replicon: chromosome
• Accession: NZ_CP046058
• Organism: Microcystis aeruginosa NIES-298

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: I4GLQ9
• Locus tag: D3800_RS08540
• Protein ID: WP_002778801.1
• Coordinates: 1768848..1769342 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: I4GLR0
• Locus tag: D3800_RS08545
• Protein ID: WP_002778803.1
• Coordinates: 1769339..1769629 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3800_RS08520	1765277..1766659	-	1383	WP_103112833.1	flavin monoamine oxidase family protein	-
D3800_RS08525	1766769..1767617	+	849	WP_002738738.1	M28 family peptidase	-
D3800_RS08530	1767843..1768217	+	375	Protein_1692	nucleotidyltransferase domain-containing protein	-
D3800_RS08535	1768214..1768696	+	483	WP_002738740.1	hypothetical protein	-
D3800_RS08540	1768848..1769342	-	495	WP_002778801.1	GNAT family N-acetyltransferase	Toxin
D3800_RS08545	1769339..1769629	-	291	WP_002778803.1	DUF1778 domain-containing protein	Antitoxin
D3800_RS08550	1769894..1770166	-	273	WP_002799473.1	hypothetical protein	-
D3800_RS08555	1770144..1770431	-	288	Protein_1697	BrnT family toxin	-
D3800_RS08560	1770659..1771156	-	498	WP_103112834.1	hypothetical protein	-
D3800_RS08565	1771230..1771640	-	411	WP_103112835.1	hypothetical protein	-
D3800_RS08570	1771760..1772167	-	408	WP_002798105.1	hypothetical protein	-
D3800_RS08575	1772652..1772978	+	327	WP_202861585.1	DUF4258 domain-containing protein	-
D3800_RS08580	1772975..1773190	+	216	WP_002757627.1	hypothetical protein	-
D3800_RS08585	1773279..1773533	-	255	WP_103112836.1	BrnA antitoxin family protein	-
D3800_RS08590	1773517..1773789	-	273	WP_016516511.1	BrnT family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1773953..1775140	1187

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17613.35 Da
• Isoelectric Point: 8.0169

>T143480 WP_002778801.1 NZ_CP046058:c1769342-1768848 [Microcystis aeruginosa NIES-298]
MNLTPPEPLASYHSITDFSCGITSLDDCLKRRAYANQASGATRTFVTCVDNRVVGYYALASGAISIQSANGKFRRNMPNP
IPVVILARLAVDTSYQGQGLGCGLFRDGALRVVKAADTIGIRGIIVHAISEEAKNFYLALGFDVSPLEPMTLMITLNDLR
ACIA

Nucleotide

• Length: 495 bp

>T143480 NZ_CP046058:c1769342-1768848 [Microcystis aeruginosa NIES-298]
ATGAATTTGACACCACCTGAACCTCTGGCTAGTTATCACTCAATCACTGATTTTTCCTGCGGAATTACTTCTCTTGATGA
TTGCTTGAAGCGTCGAGCTTATGCTAATCAAGCCAGTGGTGCAACTAGAACTTTTGTTACCTGCGTTGACAATAGGGTAG
TTGGGTATTACGCCCTTGCTTCCGGGGCGATTAGTATACAATCAGCTAATGGTAAATTTCGGCGGAATATGCCTAACCCA
ATTCCAGTGGTAATTTTGGCACGATTGGCAGTAGATACTTCTTACCAAGGTCAAGGTTTAGGATGCGGTTTATTTCGTGA
TGGTGCGCTACGGGTTGTTAAAGCGGCTGATACCATTGGTATTCGGGGAATTATTGTTCATGCTATTTCAGAAGAAGCTA
AGAATTTTTATTTGGCTTTAGGTTTTGATGTGTCTCCTCTTGAACCGATGACGTTGATGATTACTCTTAATGATTTACGC
GCTTGTATTGCTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 11041.66 Da
• Isoelectric Point: 9.7643

>AT143480 WP_002778803.1 NZ_CP046058:c1769629-1769339 [Microcystis aeruginosa NIES-298]
MSSKTKSKTPRNTLNLRIKPEERNLIDMAAKVQGKNRTDFILEAARNAAEETLLERTIFWASPEAYAEFIALLDAPPQPN
ERLRKTMQTLAPWEKE

Nucleotide

• Length: 291 bp

>AT143480 NZ_CP046058:c1769629-1769339 [Microcystis aeruginosa NIES-298]
ATGAGTAGTAAAACAAAAAGTAAGACTCCGCGTAATACCCTAAATTTGCGAATTAAGCCAGAAGAACGCAATTTGATCGA
TATGGCAGCCAAAGTGCAAGGTAAGAATAGAACTGATTTTATTTTGGAAGCCGCGCGGAATGCTGCGGAGGAAACTTTGC
TCGAACGTACTATTTTTTGGGCTAGTCCAGAGGCTTATGCAGAATTTATCGCACTTCTAGATGCACCACCTCAACCTAAT
GAACGTTTACGCAAAACCATGCAGACGCTCGCACCTTGGGAGAAGGAATGA

Structures

Toxin

• AlphaFold DB: I4GLQ9

Antitoxin

• AlphaFold DB: I4GLR0