Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-PHD
Location 1757357..1757997 Replicon chromosome
Accession NZ_CP046058
Organism Microcystis aeruginosa NIES-298

Toxin (Protein)


Gene name vapC Uniprot ID I4GXI2
Locus tag D3800_RS08470 Protein ID WP_002779922.1
Coordinates 1757357..1757755 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID S3JHM9
Locus tag D3800_RS08475 Protein ID WP_016514827.1
Coordinates 1757752..1757997 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D3800_RS08420 1752399..1752803 - 405 WP_103112826.1 hypothetical protein -
D3800_RS08425 1752903..1753292 - 390 WP_103112827.1 type II toxin-antitoxin system VapC family toxin -
D3800_RS08430 1753285..1753524 - 240 WP_002739406.1 hypothetical protein -
D3800_RS08435 1754428..1754574 - 147 Protein_1675 IS5/IS1182 family transposase -
D3800_RS08440 1754594..1755460 - 867 WP_162205091.1 IS5 family transposase -
D3800_RS08450 1755870..1756016 + 147 WP_103112828.1 PEP-CTERM sorting domain-containing protein -
D3800_RS08455 1756207..1756452 + 246 WP_002770883.1 UPF0175 family protein -
D3800_RS08460 1756449..1756934 + 486 WP_103112829.1 DUF3368 domain-containing protein -
D3800_RS08465 1756950..1757204 - 255 WP_002739141.1 hypothetical protein -
D3800_RS08470 1757357..1757755 - 399 WP_002779922.1 type II toxin-antitoxin system VapC family toxin Toxin
D3800_RS08475 1757752..1757997 - 246 WP_016514827.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
D3800_RS08480 1758354..1758833 + 480 WP_103112830.1 type II toxin-antitoxin system VapC family toxin -
D3800_RS08485 1758961..1760118 - 1158 WP_103112831.1 8-amino-7-oxononanoate synthase -
D3800_RS08490 1760186..1760545 - 360 WP_002765174.1 hypothetical protein -
D3800_RS08495 1760754..1761302 + 549 WP_002755893.1 RDD family protein -
D3800_RS08500 1761510..1761785 + 276 WP_002738957.1 hypothetical protein -
D3800_RS08510 1762273..1762464 - 192 WP_043998683.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(4-128)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-41)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15029.13 Da        Isoelectric Point: 4.5341

>T143479 WP_002779922.1 NZ_CP046058:c1757755-1757357 [Microcystis aeruginosa NIES-298]
VRLLLDTQCWLWWFVQPDKLSENVIEQIANESNEVWFSVASVWEMGIKVSIGKLPLPEQIDDYVSTRMTRLGARSLEINA
SHALRAAALPLHHRDPFDRMLIAQAQVEDMTLVSADSTFNQYEVSLLWAASS

Download         Length: 399 bp

>T143479 NZ_CP061108:2437419-2438741 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CAGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTTAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCTGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCGTTTTTGATGGGGTCCAGCGAGGCGCTTCGCGATCGTTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTAAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8858.15 Da        Isoelectric Point: 5.0190

>AT143479 WP_016514827.1 NZ_CP046058:c1757997-1757752 [Microcystis aeruginosa NIES-298]
METVNIHQAKTNLSRLLSRVELGEVIVISNRGIPIAKLVPFRTSLDRRSSLGQDRGMLTIPDDFNAPLPEDILGAFEGGA
E

Download         Length: 246 bp

>AT143479 NZ_CP061108:2437153-2437419 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACACTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB I4GXI2

Antitoxin

Source ID Structure
AlphaFold DB A0A3N0W8U9

References